CoraLite®594-conjugated EIF1 Monoclonal antibody

EIF1 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2G8C6

Cat no : CL594-67601

Synonyms

A121, EIF 1, EIF1, EIF1A, ISO1, SUI1, Sui1iso1

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Validation Data Gallery

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  IF Staining of HepG2 using CL594-67601

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®594 EIF1 antibody (CL594-67601, Clone: 2G8C6 ) at dilution of 1:200.

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  IF Staining of MCF-7 using CL594-67601

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite®594 EIF1 antibody (CL594-67601, Clone: 2G8C6 ) at dilution of 1:200.

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Tested Applications

• Positive IF/ICC detected in: MCF-7 cells, HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67601 targets EIF1 in IF/ICC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: EIF1 fusion protein Ag7843
• Full Name: eukaryotic translation initiation factor 1
• Calculated Molecular Weight: 13 kDa
• Observed Molecular Weight: 13 kDa
• GenBank Accession Number: BC005118
• Gene Symbol: EIF1
• Gene ID (NCBI): 10209
• RRID: AB_2920163
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage.

Background Information

It is a necessary for scanning and involved in initiation site selection. Promotes the assembly of 48S ribosomal complexes at the authentic initiation codon of a conventional capped mRNA. Molecular weight is 13kd.