EHD2 Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

EHD2 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 11440-1-PBS

Synonyms

---

Validation Data Gallery

• 

  WB analysis using 11440-1-AP (same clone as 11440-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11440-1-AP (EHD2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11440-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human heart using 11440-1-AP (same clone as 11440-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 11440-1-AP (EHD2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11440-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse heart using 11440-1-AP (same clone as 11440-1-PBS)

  IP result of anti-EHD2 (IP:11440-1-AP, 4ug; Detection:11440-1-AP 1:800) with mouse heart tissue lysate 4000ug. This data was developed using the same antibody clone with 11440-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 11440-1-AP (same clone as 11440-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11440-1-AP (EHD2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11440-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 11440-1-AP (same clone as 11440-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11440-1-AP (EHD2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11440-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 11440-1-AP (same clone as 11440-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 11440-1-AP (EHD2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11440-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 11440-1-AP (same clone as 11440-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 11440-1-AP (EHD2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11440-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human oesophagus cancer using 11440-1-AP (same clone as 11440-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 11440-1-AP (EHD2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11440-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human oesophagus cancer using 11440-1-AP (same clone as 11440-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 11440-1-AP (EHD2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11440-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 11440-1-AP (same clone as 11440-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11440-1-AP (EHD2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11440-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 11440-1-AP (same clone as 11440-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11440-1-AP (EHD2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11440-1-PBS in a different storage buffer formulation.

---

Product Information

11440-1-PBS targets EHD2 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EHD2 fusion protein Ag2002
• Full Name: EH-domain containing 2
• Calculated Molecular Weight: 543 aa, 61 kDa
• Observed Molecular Weight: 61 kDa
• GenBank Accession Number: BC014445
• Gene Symbol: EHD2
• Gene ID (NCBI): 30846
• RRID: AB_2097325
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.