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  • Featured Product
  • KD/KO Validated

EHD2 Polyclonal antibody

EHD2 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Cat no : 11440-1-AP

Synonyms

PAST2, PAST homolog 2, EH domain-containing protein 2, EH domain containing protein 2, EH domain containing 2

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using EHD2 Polyclonal antibody (11440-1-AP)

    WB analysis using 11440-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11440-1-AP (EHD2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using EHD2 Polyclonal antibody (11440-1-AP)

    WB analysis of human heart using 11440-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 11440-1-AP (EHD2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse heart tissue using EHD2 Polyclonal antibody (11440-1-AP)

    IP experiment of mouse heart using 11440-1-AP

    IP result of anti-EHD2 (IP:11440-1-AP, 4ug; Detection:11440-1-AP 1:800) with mouse heart tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using EHD2 Polyclonal antibody (11440-1-AP)

    IHC staining of human breast cancer using 11440-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11440-1-AP (EHD2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using EHD2 Polyclonal antibody (11440-1-AP)

    IHC staining of human breast cancer using 11440-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11440-1-AP (EHD2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human normal colon using EHD2 Polyclonal antibody (11440-1-AP)

    IHC staining of human normal colon using 11440-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 11440-1-AP (EHD2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human normal colon using EHD2 Polyclonal antibody (11440-1-AP)

    IHC staining of human normal colon using 11440-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 11440-1-AP (EHD2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using EHD2 Polyclonal antibody (11440-1-AP)

    IHC staining of human ovary tumor using 11440-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11440-1-AP (EHD2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using EHD2 Polyclonal antibody (11440-1-AP)

    IHC staining of human oesophagus cancer using 11440-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 11440-1-AP (EHD2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using EHD2 Polyclonal antibody (11440-1-AP)

    IHC staining of human oesophagus cancer using 11440-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 11440-1-AP (EHD2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using EHD2 Polyclonal antibody (11440-1-AP)

    IHC staining of human ovary tumor using 11440-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11440-1-AP (EHD2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHeLa cells, human heart tissue, mouse heart tissue, rat heart tissue
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman breast cancer tissue, human normal colon, human oesophagus cancer tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11440-1-AP targets EHD2 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EHD2 fusion protein Ag2002
Full Name EH-domain containing 2
Calculated Molecular Weight 543 aa, 61 kDa
Observed Molecular Weight 61 kDa
GenBank Accession NumberBC014445
Gene Symbol EHD2
Gene ID (NCBI) 30846
RRIDAB_2097325
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for EHD2 antibody 11440-1-APDownload protocol
IHC protocol for EHD2 antibody 11440-1-APDownload protocol
IP protocol for EHD2 antibody 11440-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Am J Cancer Res

Ese-3 contributes to colon cancer progression by downregulating EHD2 and transactivating INPP4B.

Authors - Junqiang Li
  • KD Validated
View Article
humanIF

PLoS One

Super-resolution analysis of PACSIN2 and EHD2 at caveolae.

Authors - Tamako Nishimura
View Article
humanIHC

Cancer Invest

EHD2 Overexpression Suppresses the Proliferation, Migration, and Invasion in Human Colon Cancer.

Authors - Chengqi Guan
View Article
WB

Cancer Invest

Identification of Differentially Expressed Proteins of Normal and Cancerous Human Colorectal Tissues by Liquid Chromatograph-Mass Spectrometer Based on iTRAQ Approach.

Authors - Jingjing Lv
View Article
mouseWB

Nature

Post-translational control of beige fat biogenesis by PRDM16 stabilization

Authors - Qiang Wang
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Laura (Verified Customer) (01-27-2022)

It works well for western blot with different bands but one specific around 65 kDa. It also works well for immunofluorescence of cells.

  • Applications: Western Blot, Immunofluorescence
  • Primary Antibody Dilution: 1/1000WB 1/100IF
  • Cell Tissue Type: MOVAS (Mouse Primary vascular aortic smooth muscle cells) and RPE-1 (Human retinal pigment epithelial-1)
FH

Kyosuke (Verified Customer) (06-12-2019)

It works well on HUVECs sample for Western blot.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: HUVECs