EGFR Monoclonal antibody, PBS Only (Capture)

EGFR Monoclonal Antibody for WB, IF/ICC, FC, Sandwich ELISA, Indirect ELISA, Blocking

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, FC, Sandwich ELISA, Indirect ELISA, Blocking

Conjugate

Unconjugated

CloneNo.

2C8C12

Cat no : 68643-1-PBS

Synonyms

Proto-oncogene c-ErbB-1, Epidermal growth factor receptor, EGF Receptor , EGF R, 2C8C12

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Validation Data Gallery

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  WB analysis of SCaBER using 68643-1-Ig (same clone as 68643-1-PBS)

  SCaBER cells were subjected to SDS PAGE followed by western blot with 68643-1-Ig (EGFR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68643-1-PBS in a different storage buffer formulation.

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  WB analysis of MDA-MB-468 using 68643-1-Ig (same clone as 68643-1-PBS)

  MDA-MB-468 cells were subjected to SDS PAGE followed by western blot with 68643-1-Ig (EGFR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68643-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68643-1-Ig (same clone as 68643-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68643-1-Ig (EGFR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68643-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP50069-1

  Sandwich ELISA standard curve of MP50069-1, EGFR Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68643-1-PBS. Detection antibody: 68643-2-PBS. Standard: Eg0204. Range: 0.156-10 ng/mL.

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  IF Staining of HaCaT using 68643-1-Ig (same clone as 68643-1-PBS)

  Immunofluorescent analysis of un-fixed HaCaT cells using EGFR antibody (68643-1-Ig, Clone: 2C8C12 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 68643-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 68643-1-Ig (same clone as 68643-1-PBS)

  Immunofluorescent analysis of un-fixed A431 cells using EGFR antibody (68643-1-Ig, Clone: 2C8C12 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 68643-1-PBS in a different storage buffer formulation.

• 

  FC experiment of A431 using 68643-1-Ig (same clone as 68643-1-PBS)

  1x10^6 A431 cells were surface stained with 0.2 μg EGFR Monoclonal Antibody (68643-1-Ig, Clone:2C8C12) and CoraLite488-conjugated Goat Anti-Mouse IgG(H+L) (Cat.NO. SA00013-1)(red), or 0.2 μg Mouse IgG1 Isotype Control (66360-1-Ig, Clone: T1F8D3F10) (blue). Cells were not fixed. This data was developed using the same antibody clone with 68643-1-PBS in a different storage buffer formulation.

• 

  Blocking experiment of HeLa cells using 68643-1-Ig (same clone as 68643-1-PBS)

  Human EGF (Cat.NO. HZ-1326 ) stimulates proliferation of HeLa cells in a dose-dependent manner (blue curve, refer to bottom X-left Y axis). The activity of human EGF (Cat.NO. HZ-1326 ) is blocked by mouse anti-human EGFR monoclonal antibody 68643-1-Ig at serial dose (red curve, refer to top X-right Y axis). The EC50 is typically 0.2-0.8 μg/mL at the presence of 1ng/ml EGF (Cat.NO. HZ-1326 ). This data was developed using the same antibody clone with 68643-1-PBS in a different storage buffer formulation.

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Product Information

68643-1-PBS targets EGFR as part of a matched antibody pair:

MP50069-1: 68643-1-PBS capture and 68643-2-PBS detection (validated in Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: EGFR fusion protein Ag24947
• Full Name: epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
• Calculated Molecular Weight: 134kd
• Observed Molecular Weight: 160 kDa
• GenBank Accession Number: NM_005228.5
• Gene Symbol: EGFR
• Gene ID (NCBI): 1956
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

EGFR, also named ERBB1, is a cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. The gene resides on chromosome 7p12, encoding a 170 kDa membrane-associated glycoprotein. Recent studies have shown EGFR plays a critical role in cancer development and progression, including cell proliferation, apoptosis, angiogenesis, and metastatic spread. Mutations in this gene are associated with lung cancer. When stained with live cells, EGFR can be transported into the cell interior, which is consistent with the literature report (PMID: 18084617).