EEF2 Monoclonal antibody, PBS Only

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  WB analysis using 67550-1-Ig (same clone as 67550-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67550-1-Ig (EEF2 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67550-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67550-1-Ig (same clone as 67550-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67550-1-Ig (EEF2 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67550-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 67550-1-Ig (same clone as 67550-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67550-1-Ig (EEF2 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67550-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67550-1-Ig (same clone as 67550-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67550-1-Ig (EEF2 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67550-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67550-1-Ig (same clone as 67550-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67550-1-Ig (EEF2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67550-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 67550-1-Ig (same clone as 67550-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67550-1-Ig (EEF2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67550-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67550-1-Ig (same clone as 67550-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67550-1-Ig (EEF2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67550-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 67550-1-Ig (same clone as 67550-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67550-1-Ig (EEF2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67550-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 67550-1-Ig (same clone as 67550-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67550-1-Ig (EEF2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67550-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67550-1-Ig (same clone as 67550-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 67550-1-Ig (EEF2 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67550-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67550-1-Ig (same clone as 67550-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using EEF2 antibody (67550-1-Ig, Clone: 1E4D5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67550-1-PBS in a different storage buffer formulation.

EEF2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 67550-1-PBS

Clone No.1E4D5

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Elongation factor 2, EF-2, EF2, EF 2, EC:3.6.5.-

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Product Information

67550-1-PBS targets EEF2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: EEF2 fusion protein Ag13859
• Full Name: eukaryotic translation elongation factor 2
• Calculated Molecular Weight: 858 aa, 95 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC126259
• Gene Symbol: EEF2
• Gene ID (NCBI): 1938
• RRID: AB_2882765
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P13639
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

EEF2 (Eukaryotic Elongation Factor 2), also known as EF-2 and EF2, is a crucial protein involved in the process of protein synthesis in eukaryotic cells. It plays a pivotal role in the elongation phase of translation, where it facilitates the movement of the ribosome along the mRNA strand. This movement is essential for the addition of amino acids to the growing polypeptide chain.