EEF1B2 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

EEF1B2 Monoclonal Antibody for WB, IF, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3E1C11

Cat no : 60329-1-PBS

Synonyms

EEF1B, EEF1B1, EEF1B2, EF 1 beta, EF1B, Elongation factor 1 beta

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Validation Data Gallery

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  WB analysis using 60329-1-Ig (same clone as 60329-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60329-1-Ig (EEF1B2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 60329-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 60329-1-Ig (same clone as 60329-1-PBS)

  WB result of EEF1B2 antibody (60329-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EEF1B2 transfected HeLa cells. This data was developed using the same antibody clone with 60329-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 60329-1-Ig (same clone as 60329-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 60329-1-Ig (EEF1B2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60329-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 60329-1-Ig (same clone as 60329-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 60329-1-Ig (EEF1B2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60329-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 60329-1-Ig (same clone as 60329-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed HeLa cells using 60329-1-Ig(EEF1B2 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60329-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60329-1-PBS targets EEF1B2 in WB, IF, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: EEF1B2 fusion protein Ag0135
• Full Name: eukaryotic translation elongation factor 1 beta 2
• Calculated Molecular Weight: 25 kDa
• Observed Molecular Weight: 30-34 kDa
• GenBank Accession Number: BC000211
• Gene Symbol: EEF1B2
• Gene ID (NCBI): 1933
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

In eukaryotes, the translation elongation factor eEF1A responsible for transporting amino-acylated tRNA to the ribosome forms a higher-order complex, eEF1H, with its guanine-nucleotide-exchange factor eEF1B. eEF1B consists of three subunits: eEF1B alpha, eEF1B beta and eEF1B gamma. The eEF1B2 possess the nucleotide-exchange activity. Although several models on the basis of in vitro experiments have been proposed for the macromolecular organization of the eEF1H complex, these models differ in various aspects. The human eukaryote elongation factor 1 beta 2 (eEF1B2) migrated as a 30-34 kDa protein in SDS-PAGE.