ECM1 Monoclonal antibody

ECM1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

6A12B3

Cat no : 66023-1-Ig

Synonyms

ECM1, extracellular matrix protein 1, Secretory component p85

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Validation Data Gallery

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  WB analysis of MCF-7 using 66023-1-Ig

  MCF7 cells were subjected to SDS PAGE followed by western blot with 66023-1-Ig (ECM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 66023-1-Ig

  human heart tissue were subjected to SDS PAGE followed by western blot with 66023-1-Ig (ECM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 66023-1-Ig

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 66023-1-Ig (ECM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 66023-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 66023-1-Ig(ECM1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 66023-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 66023-1-Ig(ECM1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver cancer using 66023-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 66023-1-Ig(ECM1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 66023-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 66023-1-Ig(ECM1 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: MCF7 cells, COLO 320 cells, human heart tissue
• Positive IHC detected in: human breast cancer tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

66023-1-Ig targets ECM1 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: ECM1 fusion protein Ag17955
• Full Name: extracellular matrix protein 1
• Calculated Molecular Weight: 540 aa, 61 kDa
• Observed Molecular Weight: 61 kDa
• GenBank Accession Number: BC023505
• Gene Symbol: ECM1
• Gene ID (NCBI): 1893
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Extracellular matrix protein 1 (ECM1) is a glycoprotein involved in a number of biological processes such as such as bone formation, skin differentiation, cell proliferation, and promotes angiostasis. Pathologically, ECM1 contributes to the formation and metastasis of several types of cancer including breast, thyroid and hepatocellular cancers.(21128013) It inhibits MMP9 proteolytic activity.