ECM1 Monoclonal antibody, PBS Only (Detector)

ECM1 Monoclonal Antibody for WB, IHC, Sandwich ELISA, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, Sandwich ELISA, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

6A12B3

Cat no : 66023-1-PBS

Synonyms

Secretory component p85, extracellular matrix protein 1, 6A12B3

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Validation Data Gallery

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  WB analysis of MCF-7 using 66023-1-Ig (same clone as 66023-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 66023-1-Ig (ECM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66023-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human heart using 66023-1-Ig (same clone as 66023-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 66023-1-Ig (ECM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66023-1-PBS in a different storage buffer formulation.

• 

  WB analysis of COLO 320 using 66023-1-Ig (same clone as 66023-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 66023-1-Ig (ECM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66023-1-PBS in a different storage buffer formulation.

• 

  Sandwich ELISA standard curve of MP50074-1

  Sandwich ELISA standard curve of MP50074-1, ECM1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68472-1-PBS. Detection antibody: 66023-1-PBS. Standard: Eg0533. Range: 0.156-10 ng/mL.

• 

  IHC staining of human breast cancer using 66023-1-Ig (same clone as 66023-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 66023-1-Ig(ECM1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66023-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66023-1-Ig (same clone as 66023-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 66023-1-Ig(ECM1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66023-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66023-1-Ig (same clone as 66023-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 66023-1-Ig(ECM1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66023-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66023-1-Ig (same clone as 66023-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 66023-1-Ig(ECM1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66023-1-PBS in a different storage buffer formulation.

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Product Information

66023-1-PBS targets ECM1 as part of a matched antibody pair:

MP50074-1: 68472-1-PBS capture and 66023-1-PBS detection (validated in Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: ECM1 fusion protein Ag17955
• Full Name: extracellular matrix protein 1
• Calculated Molecular Weight: 540 aa, 61 kDa
• Observed Molecular Weight: 61 kDa
• GenBank Accession Number: BC023505
• Gene Symbol: ECM1
• Gene ID (NCBI): 1893
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Extracellular matrix protein 1 (ECM1) is a glycoprotein involved in a number of biological processes such as such as bone formation, skin differentiation, cell proliferation, and promotes angiostasis. Pathologically, ECM1 contributes to the formation and metastasis of several types of cancer including breast, thyroid and hepatocellular cancers.(21128013) It inhibits MMP9 proteolytic activity.