CoraLite® Plus 488-conjugated ECHS1 Recombinant antibody

ECHS1 Recombinant Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

5G5

Cat no : CL488-81611

Synonyms

ECHS1, Enoyl CoA hydratase 1, SCEH

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Validation Data Gallery

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  IF Staining of HeLa using CL488-81611

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 ECHS1 antibody (CL488-81611, Clone: 5G5 ) at dilution of 1:200, CL594-Phalloidin (red).

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Tested Applications

• Positive IF detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-81611 targets ECHS1 in IF applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ECHS1 fusion protein Ag1842
• Full Name: enoyl Coenzyme A hydratase, short chain, 1, mitochondrial
• Calculated Molecular Weight: 31 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC008906
• Gene Symbol: ECHS1
• Gene ID (NCBI): 1892
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Enoyl-coenzyme A hydratase (ECHS1) is a mitochondrial protein which catalyzes the hydration of 2-trans-enoyl-coenzyme A intermediates to L-3-hydroxyacyl-coenzyme A, playing a key role in metabolism of fatty acids in mitochondria. ECHS1 is highly expressed in muscle, liver and fibroblasts. Altered expression of ECHS1 has been considered a characteristic feature of mitochondria dysfunction. (23275097, 23235493)