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E2F8 Polyclonal antibody, PBS Only

E2F8 Polyclonal Antibody for WB, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 13425-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using E2F8 Polyclonal antibody (13425-1-AP)

    WB analysis of HeLa using 13425-1-AP (same clone as 13425-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 13425-1-AP (E2F8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13425-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using E2F8 Polyclonal antibody (13425-1-AP)

    WB analysis of HEK-293 using 13425-1-AP (same clone as 13425-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 13425-1-AP (E2F8 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13425-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using E2F8 Polyclonal antibody (13425-1-AP)

    IP experiment of HEK-293 using 13425-1-AP (same clone as 13425-1-PBS)

    IP result of anti-E2F8 (IP:13425-1-AP, 4ug; Detection:13425-1-AP 1:500) with HEK-293 cells lysate 2800ug. This data was developed using the same antibody clone with 13425-1-PBS in a different storage buffer formulation.

Product Information

13425-1-PBS targets E2F8 in WB, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen E2F8 fusion protein Ag4216
Full Name E2F transcription factor 8
Calculated Molecular Weight 867 aa, 94 kDa
Observed Molecular Weight 105 kDa
GenBank Accession NumberBC028244
Gene Symbol E2F8
Gene ID (NCBI) 79733
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

E2F8 is one E2F transcription factor that is essential for orchestrating expression of genes required for cell cycle progression, proliferation, apoptosis and differentiation. E2F8 shows a high degree of resemblance to E2F7 and shares the unique structure of E2F7 by having two distinct domains exhibiting a high degree of similarity to the DNA-binding domain of the E2F familyTogether with E2F7, they possess two DNA-binding domains that are predicted to interact with each other. E2F8 binds consensus E2F sites in a DP-independent manner and represses transcription of E2F-regulated promoters. Ectopic expression of E2F8 inhibits cellular proliferation. The calcualted molecular weight of E2F8 is 94 kDa, but modified E2F8 is about 105 kDa. (PMID: 15897886)