E2F8 Polyclonal antibody, PBS Only

E2F8 Polyclonal Antibody for WB, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 13425-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of HeLa using 13425-1-AP (same clone as 13425-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 13425-1-AP (E2F8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13425-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 13425-1-AP (same clone as 13425-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13425-1-AP (E2F8 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13425-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HEK-293 using 13425-1-AP (same clone as 13425-1-PBS)

  IP result of anti-E2F8 (IP:13425-1-AP, 4ug; Detection:13425-1-AP 1:500) with HEK-293 cells lysate 2800ug. This data was developed using the same antibody clone with 13425-1-PBS in a different storage buffer formulation.

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Product Information

13425-1-PBS targets E2F8 in WB, IP, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: E2F8 fusion protein Ag4216
• Full Name: E2F transcription factor 8
• Calculated Molecular Weight: 867 aa, 94 kDa
• Observed Molecular Weight: 105 kDa
• GenBank Accession Number: BC028244
• Gene Symbol: E2F8
• Gene ID (NCBI): 79733
• RRID: AB_2097273
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

E2F8 is one E2F transcription factor that is essential for orchestrating expression of genes required for cell cycle progression, proliferation, apoptosis and differentiation. E2F8 shows a high degree of resemblance to E2F7 and shares the unique structure of E2F7 by having two distinct domains exhibiting a high degree of similarity to the DNA-binding domain of the E2F familyTogether with E2F7, they possess two DNA-binding domains that are predicted to interact with each other. E2F8 binds consensus E2F sites in a DP-independent manner and represses transcription of E2F-regulated promoters. Ectopic expression of E2F8 inhibits cellular proliferation. The calcualted molecular weight of E2F8 is 94 kDa, but modified E2F8 is about 105 kDa. (PMID: 15897886)