E2F8 Polyclonal antibody

E2F8 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 13425-1-AP

Synonyms

E2F 8, E2F transcription factor 8, E2F8, Transcription factor E2F8



Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells
Positive IP detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13425-1-AP targets E2F8 in WB, IP, ChIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, goat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen E2F8 fusion protein Ag4216
Full Name E2F transcription factor 8
Calculated Molecular Weight 867 aa, 94 kDa
Observed Molecular Weight 105 kDa
GenBank Accession NumberBC028244
Gene Symbol E2F8
Gene ID (NCBI) 79733
RRIDAB_2097273
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

E2F8 is one E2F transcription factor that is essential for orchestrating expression of genes required for cell cycle progression, proliferation, apoptosis and differentiation. E2F8 shows a high degree of resemblance to E2F7 and shares the unique structure of E2F7 by having two distinct domains exhibiting a high degree of similarity to the DNA-binding domain of the E2F familyTogether with E2F7, they possess two DNA-binding domains that are predicted to interact with each other. E2F8 binds consensus E2F sites in a DP-independent manner and represses transcription of E2F-regulated promoters. Ectopic expression of E2F8 inhibits cellular proliferation. The calcualted molecular weight of E2F8 is 94 kDa, but modified E2F8 is about 105 kDa. (PMID: 15897886)

Protocols

Product Specific Protocols
WB protocol for E2F8 antibody 13425-1-APDownload protocol
IP protocol for E2F8 antibody 13425-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Int J Cancer

HOXD-AS1/miR-130a sponge regulates glioma development by targeting E2F8.

Authors - Yinan Chen
goatWB

Cell Death Discov

Molecular mechanism underlying miR-204-5p regulation of adipose-derived stem cells differentiation into cells from three germ layers

Authors - Zhimin Wang
humanWB,ChIP

Adv Sci (Weinh)

Genetic Aberrations and Interaction of NEK2 and TP53 Accelerate Aggressiveness of Multiple Myeloma

Authors - Xiangling Feng
humanChIP

Int J Toxicol

Transcription Factor E2F8 Promotes Cisplatin Resistance in Hepatocellular Carcinoma by Regulating DNA Damage via NUSAP1

Authors - Jianqiao Kong
humanWB

Cell Death Discov

Carmofur prevents cell cycle progression by reducing E2F8 transcription in temozolomide-resistant glioblastoma cells

Authors - Cyntanna C Hawkins