E-selectin/CD62E Polyclonal antibody, PBS Only

E-selectin/CD62E Polyclonal Antibody for IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 20894-1-PBS

Synonyms

CD62E, E-selectin, CD62 antigen-like family member E, ELAM1, ELAM 1

---

Validation Data Gallery

• 

  IHC staining of human tonsillitis using 20894-1-AP (same clone as 20894-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 20894-1-AP (E-selectin / CD62E antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20894-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 20894-1-AP (same clone as 20894-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 20894-1-AP (E-selectin / CD62E antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20894-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human appendicitis using 20894-1-AP (same clone as 20894-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 20894-1-AP (E-selectin /CD62E antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20894-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human appendicitis using 20894-1-AP (same clone as 20894-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 20894-1-AP (E-selectin /CD62E antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20894-1-PBS in a different storage buffer formulation.

---

Product Information

20894-1-PBS targets E-selectin/CD62E in IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: E-selectin/CD62E fusion protein Ag14944
• Full Name: selectin E
• Calculated Molecular Weight: 610 aa, 67 kDa
• GenBank Accession Number: BC142711
• Gene Symbol: CD62E
• Gene ID (NCBI): 6401
• RRID: AB_10755287
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

E-selectin, also known as ELAM-1 or CD62E, is a member of the selectin family of adhesion molecules that also includes L-selectin and P-selectin. E-selectin is an inducible endothelial cell surface molecule. Its expression on endothelial cells is transcriptionally upregulated by various proinflammatory substances such as IL-1, TNFα and lipopolysaccharide (LPS). Besides, it can be secreted by endothelial cells, and can be detected in a soluble form (sE-selectin) in serum. During inflammation, E-selectin plays an important part in recruiting leukocytes to the site of injury. (PMID: 2827173; 1382417; 8943958)