Anti-Mouse CD324 (E-cadherin) Rabbit Recombinant Antibody

E-Cadherin Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

mouse, canine

Applications

FC

Conjugate

Unconjugated

CloneNo.

241282C1

Cat no : 98140-1-RR

Synonyms

Um, L CAM, Ecad, Cdh1, cadherin 1

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Validation Data Gallery

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  FC experiment of MDCK using 98140-1-RR

  1x10^6 MDCK cells were surface stained with 0.25 ug Anti-Mouse E-Cadherin Rabbit Recombinant Antibody (98140-1-RR, Clone:241282C1) (red) or Isotype Control (blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

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  Affinity and Kinetic Characterization of 98140-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98140-1-RR against Mouse E-Cadherin were performed. The affinity constant is 3.67 nM.

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Tested Applications

• Positive FC detected in: MDCK cells

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 98140-1-RR is E-Cadherin Fusion Protein expressed in E. coli.

• Tested Reactivity: mouse, canine
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: cadherin 1
• Calculated Molecular Weight: 98kDa
• GenBank Accession Number: NM_009864.3
• Gene Symbol: E-cadherin
• Gene ID (NCBI): 12550
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. E-cadherin (epithelial cadherin), also known as CD324, CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness.