Anti-Mouse E-Cadherin Rabbit Recombinant Antibody, PBS Only

E-Cadherin Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

mouse, canine

Applications

FC

Conjugate

Unconjugated

CloneNo.

241282C1

Cat no : 98140-1-PBS

Synonyms

Um, L CAM, Ecad, Cdh1, cadherin 1

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Validation Data Gallery

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  FC experiment of MDCK using 98140-1-RR (same clone as 98140-1-PBS)

  1x10^6 MDCK cells were surface stained with 0.25 ug Anti-Mouse E-Cadherin Rabbit Recombinant Antibody (98140-1-RR, Clone:241282C1) (red) or Isotype Control (blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98140-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 98140-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98140-1-RR against Mouse E-Cadherin were performed. The affinity constant is 3.67 nM.

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Product Information

The immunogen of 98140-1-PBS is E-Cadherin Fusion Protein expressed in E. coli.

• Tested Reactivity: mouse, canine
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: cadherin 1
• Calculated Molecular Weight: 98kDa
• GenBank Accession Number: NM_009864.3
• Gene Symbol: E-cadherin
• Gene ID (NCBI): 12550
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. E-cadherin (epithelial cadherin), also known as CD324, CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness.