CoraLite® Plus 488-conjugated Dystrophin Monoclonal antibody

Dystrophin Monoclonal Antibody for IF/ICC, IF-P, FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

IF/ICC, IF-P, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1G12E6

Cat no : CL488-68120

Synonyms

DMD, BMD, 1G12E6



Tested Applications

Positive IF-P detected inmouse skeletal muscle tissue
Positive IF/ICC detected inH9C2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68120 targets Dystrophin in IF/ICC, IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Dystrophin fusion protein Ag4392
Full Name dystrophin
Calculated Molecular Weight 3685 aa, 427 kDa
Observed Molecular Weight70 kDa, 430 kDa
GenBank Accession NumberBC028720
Gene Symbol Dystrophin
Gene ID (NCBI) 1756
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Dystrophin (DMD or BMD) is a large muscle protein whose mutations cause Duchenne muscular dystrophy (DMD) and Becker muscular dystrophy (BMD), the childhood neuromuscular disorders that result in progressive muscle weakness, respiratory difficulties and cardiovascular dysfunction. Dystrophin is a crucial component of the dystrophin-glycoprotein complex which is essential for muscle membrane integrity and stability. Dystrophin is located on the cytoplasmic face of the sarcolemma and connects the cytoskeletal network to the sarcolemma and extracellular matrix. Multiple isoforms of dystrophin exist due to the alternative splicing, with a wide range of MW (69-72, 110-143, 271, 426 kDa). Most tissues contain transcripts of several isoforms.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 Dystrophin antibody CL488-68120Download protocol
Standard Protocols
Click here to view our Standard Protocols