Various lysates were subjected to SDS PAGE followed by western blot with 68120-1-Ig (Dystrophin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 68120-1-Ig (Dystrophin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 68120-1-Ig
HeLa cells were subjected to SDS PAGE followed by western blot with 68120-1-Ig (Dystrophin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
HeLa cells were subjected to SDS PAGE followed by western blot with 68120-1-Ig (Dystrophin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of rat heart using 68120-1-Ig
rat heart tissue were subjected to SDS PAGE followed by western blot with 68120-1-Ig (Dystrophin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
rat heart tissue were subjected to SDS PAGE followed by western blot with 68120-1-Ig (Dystrophin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis using 68120-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 68120-1-Ig (DMD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 68120-1-Ig (DMD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IHC staining of rat skeletal muscle using 68120-1-Ig
Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 68120-1-Ig (Dystrophin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 68120-1-Ig (Dystrophin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse skeletal muscle using 68120-1-Ig
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 68120-1-Ig (Dystrophin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using Dystrophin antibody (68120-1-Ig, Clone: 1G12E6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
IF Staining of rat heart using 68120-1-Ig
Immunofluorescent analysis of (4% PFA) fixed rat heart tissue using Dystrophin antibody (68120-1-Ig, Clone: 1G12E6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
Immunofluorescent analysis of (4% PFA) fixed rat heart tissue using Dystrophin antibody (68120-1-Ig, Clone: 1G12E6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
FC experiment of HepG2 using 68120-1-Ig
1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human Dystrophin (68120-1-Ig, Clone:1G12E6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human Dystrophin (68120-1-Ig, Clone:1G12E6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
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rat brain tissue, HeLa cells, rat heart tissue, HepG2 cells, mouse brain tissue
Positive IHC detected in
rat skeletal muscle tissue, mouse skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected in
mouse skeletal muscle tissue, rat heart tissue
Positive FC (Intra) detected in
HepG2 cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:5000-1:50000
Immunohistochemistry (IHC)
IHC : 1:1000-1:4000
Immunofluorescence (IF)-P
IF-P : 1:200-1:800
Flow Cytometry (FC) (INTRA)
FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
Dystrophin (DMD or BMD) is a large muscle protein whose mutations cause Duchenne muscular dystrophy (DMD) and Becker muscular dystrophy (BMD), the childhood neuromuscular disorders that result in progressive muscle weakness, respiratory difficulties and cardiovascular dysfunction. Dystrophin is a crucial component of the dystrophin-glycoprotein complex which is essential for muscle membrane integrity and stability. Dystrophin is located on the cytoplasmic face of the sarcolemma and connects the cytoskeletal network to the sarcolemma and extracellular matrix. Multiple isoforms of dystrophin exist due to the alternative splicing, with a wide range of MW (69-72, 110-143, 271, 426 kDa). Most tissues contain transcripts of several isoforms.
Various lysates were subjected to SDS PAGE followed by western blot with 68120-1-Ig (Dystrophin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 68120-1-Ig
HeLa cells were subjected to SDS PAGE followed by western blot with 68120-1-Ig (Dystrophin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of rat heart using 68120-1-Ig
rat heart tissue were subjected to SDS PAGE followed by western blot with 68120-1-Ig (Dystrophin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis using 68120-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 68120-1-Ig (DMD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of rat skeletal muscle using 68120-1-Ig
Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 68120-1-Ig (Dystrophin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse skeletal muscle using 68120-1-Ig
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 68120-1-Ig (Dystrophin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse skeletal muscle using 68120-1-Ig
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 68120-1-Ig (Dystrophin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF-P Figures
IF Staining of mouse skeletal muscle using 68120-1-Ig
Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using Dystrophin antibody (68120-1-Ig, Clone: 1G12E6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
IF Staining of rat heart using 68120-1-Ig
Immunofluorescent analysis of (4% PFA) fixed rat heart tissue using Dystrophin antibody (68120-1-Ig, Clone: 1G12E6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
FC (INTRA) Figures
FC experiment of HepG2 using 68120-1-Ig
1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human Dystrophin (68120-1-Ig, Clone:1G12E6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
Dystrophin Monoclonal antibody
Catalog Number
68120-1-Ig
Citations
2
Dilutions
WB : 1:5000-1:50000 IHC : 1:1000-1:4000 IF-P : 1:200-1:800 FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Applications
WB, IHC, IF-P, FC (Intra), ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
