Dystroglycan Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

Dystroglycan Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2B1G12

Cat no : 66735-1-PBS

Synonyms

DAG1, DAG, Beta DG, Alpha DG Beta dystroglycan, AGRNR

---

Validation Data Gallery

• 

  WB analysis using 66735-1-Ig (same clone as 66735-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66735-1-Ig (Dystroglycan antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66735-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 66735-1-Ig (same clone as 66735-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66735-1-Ig (Dystroglycan antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66735-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 66735-1-Ig (same clone as 66735-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66735-1-Ig (Dystroglycan antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66735-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skeletal muscle using 66735-1-Ig (same clone as 66735-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 66735-1-Ig (Dystroglycan antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66735-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skeletal muscle using 66735-1-Ig (same clone as 66735-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 66735-1-Ig (Dystroglycan antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66735-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat heart using 66735-1-Ig (same clone as 66735-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 66735-1-Ig (Dystroglycan antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66735-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat heart using 66735-1-Ig (same clone as 66735-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 66735-1-Ig (Dystroglycan antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66735-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat skeletal muscle using 66735-1-Ig (same clone as 66735-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 66735-1-Ig (Dystroglycan antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66735-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat skeletal muscle using 66735-1-Ig (same clone as 66735-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 66735-1-Ig (Dystroglycan antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66735-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 66735-1-Ig (same clone as 66735-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Dystroglycan antibody (66735-1-Ig, Clone: 2B1G12 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66735-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A549 using 66735-1-Ig (same clone as 66735-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using Dystroglycan antibody (66735-1-Ig, Clone: 2B1G12 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 66735-1-PBS in a different storage buffer formulation.

---

Product Information

66735-1-PBS targets Dystroglycan in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Dystroglycan fusion protein Ag27222
• Full Name: dystroglycan 1 (dystrophin-associated glycoprotein 1)
• Calculated Molecular Weight: 97 kDa
• Observed Molecular Weight: 43 kDa, 30 kDa
• GenBank Accession Number: BC012740
• Gene Symbol: Dystroglycan
• Gene ID (NCBI): 1605
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Dystroglycan, also known as DAG1 or DG, was originally isolated from skeletal muscle as an integral membrane component of the dystrophin-glycoprotein complex (DGC). In addition to skeletal muscle, dystroglycan is strongly expressed in heart and smooth muscle, as well as many non-muscle tissues including brain and peripheral nerve (PMID: 12556455). The dystroglycan is involved in a number of processes including laminin and basement membrane assembly, sarcolemmal stability, cell survival, peripheral nerve myelination, nodal structure, cell migration, and epithelial polarization. Dystroglycan consists of two subunits (alpha and beta), which are translated from a single mRNA as a propeptide that is proteolytically cleaved into two noncovalently associated proteins (PMID: 16410545). Alpha-dystroglycan is a 156-kDa extracellular peripheral glycoprotein, while beta-dystroglycan is a 43-kDa transmembrane protein (PMID: 9858474). The 43-kDa beta-dystroglycan can be cleaved into a ~30-kDa form (PMID: 14678802; 18458097; 17255331).