Drebrin Recombinant antibody, PBS Only (Capture)

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  WB analysis using 83591-2-RR (same clone as 83591-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83591-2-RR (Drebrin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83591-2-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 83591-2-RR (same clone as 83591-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83591-2-RR (Drebrin antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9). This data was developed using the same antibody clone with 83591-2-PBS in a different storage buffer formulation.

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  FC experiment of SH-SY5Y using 83591-2-RR (same clone as 83591-2-PBS)

  1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug Drebrin Recombinant antibody (83591-2-RR, Clone:240599G12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83591-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00585-2

  Cytometric bead array standard curve of MP00585-2, Drebrin Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83591-2-PBS. Detection antibody: 83591-1-PBS. Standard: Ag0371. Range: 0.78-100 ng/mL.

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  Affinity and Kinetic Characterization of 83591-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83591-2-RR against Human DHRS3 were performed. The affinity constant is 0.109 nM.

Drebrin {ptg:Brand} Recombinant Antibody for WB, IHC, FC (Intra), Cytometric bead array, Indirect ELISA

Cat No. 83591-2-PBS

Clone No.240599G12

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), Cytometric bead array, Indirect ELISA

Synonyms

DBN1, drebrin 1, Developmentally-regulated brain protein, DBN 1, 240599G12

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Product Information

83591-2-PBS targets Drebrin as part of a matched antibody pair:

MP00585-2: 83591-2-PBS capture and 83591-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Drebrin fusion protein Ag0371
• Full Name: drebrin 1
• Calculated Molecular Weight: 71 kDa
• Observed Molecular Weight: 110-130 kDa
• GenBank Accession Number: BC000283
• Gene Symbol: Drebrin
• Gene ID (NCBI): 1627
• ENSEMBL Gene ID: ENSG00000113758
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q16643
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

Drebrin (DBN1) is an actin binding and stabilizing protein with roles in endocytosis, formation of dendrite spines in neurons and coordinating cell-cell synapses in immune cells. Preferentially expressed in the brain, it is highly localized in dendritic spines and regulates spine shapes. It has two isoforms that are splice variants, with drebrin A being expressed in neural cells, and drebrin E is present in several other cell types. Recently it has been reported that circulating DBN1 levels correlated with retinal nerve fiber layer defect and may reflect the severity of retinal ganglion cells injury in glaucoma patients.