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Drebrin Recombinant antibody

Drebrin Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240599G12

Cat no : 83591-2-RR

Synonyms

DBN1, drebrin 1, Developmentally-regulated brain protein, DBN 1, 240599G12

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Drebrin Recombinant antibody (83591-2-RR)

    WB analysis using 83591-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83591-2-RR (Drebrin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of SH-SY5Y cells using Drebrin Recombinant antibody (83591-2-RR)

    FC experiment of SH-SY5Y using 83591-2-RR

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug Drebrin Recombinant antibody (83591-2-RR, Clone:240599G12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83591-2-RR

    Affinity and Kinetic Characterization of 83591-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83591-2-RR against Human DHRS3 were performed. The affinity constant is 0.109 nM.

Tested Applications

Positive WB detected inHeLa cells, K-562 cells, Neuro-2a cells, SH-SY5Y cells, PC-12 cells
Positive FC (Intra) detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83591-2-RR is Drebrin Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Drebrin fusion protein Ag0371
Full Name drebrin 1
Calculated Molecular Weight 71 kDa
Observed Molecular Weight 110-130 kDa
GenBank Accession NumberBC000283
Gene Symbol Drebrin
Gene ID (NCBI) 1627
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Drebrin (DBN1) is an actin binding and stabilizing protein with roles in endocytosis, formation of dendrite spines in neurons and coordinating cell-cell synapses in immune cells. Preferentially expressed in the brain, it is highly localized in dendritic spines and regulates spine shapes. It has two isoforms that are splice variants, with drebrin A being expressed in neural cells, and drebrin E is present in several other cell types. Recently it has been reported that circulating DBN1 levels correlated with retinal nerve fiber layer defect and may reflect the severity of retinal ganglion cells injury in glaucoma patients.

Protocols

Product Specific Protocols
WB protocol for Drebrin antibody 83591-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols