Di-Methyl-Histone H3 (Arg2) Recombinant antibody

Di-Methyl-Histone H3 (Arg2) Uni-rAb^TM Recombinant Antibody for IF/ICC, FC (Intra), Dot Blot, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), Dot Blot, ELISA

Conjugate

Unconjugated

CloneNo.

241896D7

Cat no : 84702-1-RR

Synonyms

Di-Methyl-Histone H3, Di-Methyl Histone-H3 Arg2, Di-Methyl Histone H3 Arg2, Di-Methyl Histone H3 (Arg2), Di Methyl Histone H3

---

Validation Data Gallery

• 

  IF Staining of HeLa using 84702-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HIST1H3A antibody (84702-1-RR, Clone: 241896D7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  FC experiment of HeLa using 84702-1-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug Hist1h3a Recombinant Antibody (84702-1-RR, Clone:241896D7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

• 

  Dot Blot experiment of peptide using 84702-1-RR

  Dot blot analysis was used to confirm the specificity of 84702-1-RR Di-Methyl-Histone H3 (Arg2) antibody. Peptides were spotted onto NC and probed with antibody at 1 µg/ml. The amount of peptide (ug/mL) spotted is indicated next to each row.

---

Tested Applications

• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HeLa cells
• Positive Dot Blot detected in: peptide

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
• DOT BLOT: DOT BLOT : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84702-1-RR targets Di-Methyl-Histone H3 (Arg2) in IF/ICC, FC (Intra), Dot Blot, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: histone cluster 1, H3a
• GenBank Accession Number: BC066245
• Gene Symbol: HIST1H3A
• Gene ID (NCBI): 8350
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.