CoraLite® Plus 488-conjugated Destrin Monoclonal antibody

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  FC experiment of MCF-7 using CL488-67657

  1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Destrin (CL488-67657, Clone:1A12D9) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Destrin Monoclonal Antibody for FC (Intra)

Cat No. CL488-67657

Clone No.1A12D9

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig

Applications

FC (Intra)

Synonyms

ACTDP, Actin depolymerizing factor, ADF, bA462D18.2, Destrin, DSN, DSTN

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Tested Applications

• Positive FC (Intra) detected in: MCF-7 cells
• Positive FC detected in: MCF-7 cells

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67657 targets Destrin in FC (Intra) applications and shows reactivity with Human, mouse, rat, pig samples.

• Tested Reactivity: Human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Destrin fusion protein Ag1402
• Full Name: destrin (actin depolymerizing factor)
• Calculated Molecular Weight: 19 kDa
• Observed Molecular Weight: 18-19 kDa
• GenBank Accession Number: BC009477
• Gene Symbol: Destrin
• Gene ID (NCBI): 11034
• RRID: AB_2923779
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P60981
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Destrin (also known as actin depolymerization factor, ADF) is an actin-binding protein contributing to cytoskeletal dynamics by promoting rapid actin filament disassembly. By regulating actin cytoskeletal reorganization it plays an important role in the formation of filopodia, which further promotes tumor cell invasion and metastasis. Destrin has been reported to be overexpressed in primary colon cancer.