DYNC2H1 Polyclonal antibody, PBS Only

DYNC2H1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 29758-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of mouse testis using 29758-1-AP (same clone as 29758-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 29758-1-AP (DYNC2H1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29758-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 29758-1-AP (same clone as 29758-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 29758-1-AP (DYNC2H1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29758-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 29758-1-AP (same clone as 29758-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 29758-1-AP (DYNC2H1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29758-1-PBS in a different storage buffer formulation.

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  IF Staining of hTERT-RPE1 using 29758-1-AP (same clone as 29758-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using DYNC2H1 antibody (29758-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite® Plus 488 ARL13B antibody (CL488-17711, green). This data was developed using the same antibody clone with 29758-1-PBS in a different storage buffer formulation.

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  IF Staining of hTERT-RPE1 using 29758-1-AP (same clone as 29758-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using DYNC2H1 antibody (29758-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite® Plus 488 ARL13B antibody (CL488-17711, green). This data was developed using the same antibody clone with 29758-1-PBS in a different storage buffer formulation.

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Product Information

29758-1-PBS targets DYNC2H1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DYNC2H1 fusion protein Ag30707
• Full Name: dynein, cytoplasmic 2, heavy chain 1
• Calculated Molecular Weight: 493 kDa
• Observed Molecular Weight: 493 kDa
• GenBank Accession Number: NM_001377
• Gene Symbol: DYNC2H1
• Gene ID (NCBI): 79659
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

DYNC2H1 (Cytoplasmic dynein 2 heavy chain 1) is involved in ciliary intraflagellar transport (IFT). DYNC2H1 drives retrograde transport of the IFT-A protein complex that regulates tip-to-base transport in cilia, involved in the generation and maintenance of cilia. DYNC2H1 variants or retina-predominant variants cause nonsyndromic retinal degeneration (PMID:32753734). DYNC2H1 mutations also cause asphyxiating thoracic dystrophy and short rib-polydactyly syndrome (PMID: 19442771). DYNC2H1 was localized to the basal bodies by immunofluorescence staining (PMID: 21552265, PMID: 26077881, PMID: 30320547).