HRP-conjugated DYKDDDDK tag Recombinant antibody (Binds to FLAG® tag epitope)

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  WB analysis using HRP-80801-2

  Various lysates were subjected to SDS PAGE followed by western blot with HRP-80801-2 (DYKDDDDK tag antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

DYKDDDDK tag Recombinant Antibody for WB

Cat No. HRP-80801-2

Clone No.240568A11

Host / Isotype

Rabbit / IgG

Reactivity

recombinant protein

Applications

WB

Synonyms

240568A11

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Tested Applications

• Positive WB detected in: Transfected HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

HRP-80801-2 targets DYKDDDDK tag in WB applications and shows reactivity with recombinant protein samples.

• Tested Reactivity: recombinant protein
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: DYKDDDDK tag fusion protein Ag2329
• Full Name: DYKDDDDK tag
• RRID: AB_3673983
• Conjugate: HRP
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The DYKDDDDK(FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal DDDDK vectors provide an Ek cleavage site for removal of the fusion tag. The DDDDK peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the DDDDK peptide is more likely to be accessible to antibodies. A DDDDK-tag can be used in many different assays that require recognition by an antibody, such as western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization and so on. This antibody is a rabbit polyclonal antibody raised against 3xFlag (3xDYKDDDDKT) sequence and recognizes the (1x) and (3x)DYKDDDDK peptide and detects DDDDK-tagged proteins. Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology.