DVL2 Monoclonal antibody, PBS Only

DVL2 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2F2D2

Cat no : 67105-1-PBS

Synonyms

Dishevelled 2, DSH homolog 2, DVL2

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Validation Data Gallery

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  WB analysis using 67105-1-Ig (same clone as 67105-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67105-1-Ig (DVL2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67105-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67105-1-Ig (same clone as 67105-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67105-1-Ig (DVL2 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67105-1-PBS in a different storage buffer formulation.

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  WB analysis of T-47D using 67105-1-Ig (same clone as 67105-1-PBS)

  T-47D cells were subjected to SDS PAGE followed by western blot with 67105-1-Ig (DVL2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67105-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 67105-1-Ig (same clone as 67105-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 67105-1-Ig (DVL2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67105-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse colon using 67105-1-Ig (same clone as 67105-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 67105-1-Ig (DVL2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67105-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse colon using 67105-1-Ig (same clone as 67105-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 67105-1-Ig (DVL2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67105-1-PBS in a different storage buffer formulation.

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  IHC staining of rat colon using 67105-1-Ig (same clone as 67105-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 67105-1-Ig (DVL2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67105-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat colon using 67105-1-Ig (same clone as 67105-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 67105-1-Ig (DVL2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67105-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 67105-1-Ig (same clone as 67105-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 67105-1-Ig (DVL2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67105-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67105-1-Ig (same clone as 67105-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67105-1-Ig (DVL2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67105-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 67105-1-Ig (same clone as 67105-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67105-1-Ig (DVL2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67105-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 67105-1-Ig (same clone as 67105-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67105-1-Ig (DVL2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67105-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 67105-1-Ig (same clone as 67105-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67105-1-Ig (DVL2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67105-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 67105-1-Ig (same clone as 67105-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 67105-1-Ig (DVL2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67105-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67105-1-PBS targets DVL2 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: DVL2 fusion protein Ag28634
• Full Name: dishevelled, dsh homolog 2 (Drosophila)
• Calculated Molecular Weight: 736 aa, 79 kDa
• Observed Molecular Weight: 90 kDa
• GenBank Accession Number: BC014844
• Gene Symbol: DVL2
• Gene ID (NCBI): 1856
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.