DVL2 Monoclonal antibody

DVL2 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

2

CloneNo.

2F2D2

Cat no : 67105-1-Ig

Synonyms

Dishevelled 2, DSH homolog 2, DVL2

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Validation Data Gallery

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  WB analysis using 67105-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67105-1-Ig (DVL2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 67105-1-Ig

  A431 cells were subjected to SDS PAGE followed by western blot with 67105-1-Ig (DVL2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67105-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67105-1-Ig (DVL2 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

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  WB analysis of T-47D using 67105-1-Ig

  T-47D cells were subjected to SDS PAGE followed by western blot with 67105-1-Ig (DVL2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse colon using 67105-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 67105-1-Ig (DVL2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 67105-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 67105-1-Ig (DVL2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat colon using 67105-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 67105-1-Ig (DVL2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat colon using 67105-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 67105-1-Ig (DVL2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 67105-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67105-1-Ig (DVL2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 67105-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67105-1-Ig (DVL2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 67105-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67105-1-Ig (DVL2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 67105-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67105-1-Ig (DVL2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 67105-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 67105-1-Ig (DVL2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 67105-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 67105-1-Ig (DVL2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: MCF-7 cells, A431 cells, T-47D cells, THP-1 cells, K-562 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells
• Positive IHC detected in: mouse colon tissue, human colon cancer tissue, human gliomas tissue, human prostate cancer tissue, rat colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:2000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IP: See 1 publications below

Product Information

67105-1-Ig targets DVL2 in WB, IHC, IP, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Cited Reactivity: human, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: DVL2 fusion protein Ag28634
• Full Name: dishevelled, dsh homolog 2 (Drosophila)
• Calculated Molecular Weight: 736 aa, 79 kDa
• Observed Molecular Weight: 90 kDa
• GenBank Accession Number: BC014844
• Gene Symbol: DVL2
• Gene ID (NCBI): 1856
• RRID: AB_2882409
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IP	Int J Biol Sci; CYR61 Acts as an Intracellular Microtubule-Associated Protein and Coordinates Mitotic Progression via PLK1-FBW7 Pathway; Authors - Kaishun Hu
rat	WB	Biochim Biophys Acta Mol Cell Res; Wnt/β-catenin signaling inhibits oxidative stress-induced ferroptosis to improve interstitial cystitis/bladder pain syndrome by reducing NF-κB; Authors - Weilin Fang