DVL1 Monoclonal antibody, PBS Only

DVL1 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A2A5

Cat no : 67672-1-PBS

Synonyms

Dishevelled 1, DSH homolog 1, DVL, DVL1

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Validation Data Gallery

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  WB analysis using 67672-1-Ig (same clone as 67672-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67672-1-Ig (DVL1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67672-1-PBS in a different storage buffer formulation.

• 

  WB analysis of ROS1728 using 67672-1-Ig (same clone as 67672-1-PBS)

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 67672-1-Ig (DVL1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67672-1-PBS in a different storage buffer formulation.

• 

  WB analysis of NCCIT using 67672-1-Ig (same clone as 67672-1-PBS)

  NCCIT cells were subjected to SDS PAGE followed by western blot with 67672-1-Ig (DVL1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67672-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat colon using 67672-1-Ig (same clone as 67672-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 67672-1-Ig (DVL1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67672-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat colon using 67672-1-Ig (same clone as 67672-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 67672-1-Ig (DVL1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67672-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse colon using 67672-1-Ig (same clone as 67672-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 67672-1-Ig (DVL1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67672-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse colon using 67672-1-Ig (same clone as 67672-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 67672-1-Ig (DVL1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67672-1-PBS in a different storage buffer formulation.

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Product Information

67672-1-PBS targets DVL1 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, rat, mouse samples.

• Tested Reactivity: Human, rat, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: DVL1 fusion protein Ag26083
• Full Name: dishevelled, dsh homolog 1 (Drosophila)
• Calculated Molecular Weight: 75 kDa
• Observed Molecular Weight: 70-75 kDa
• GenBank Accession Number: BC050454
• Gene Symbol: DVL1
• Gene ID (NCBI): 1855
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

DVL1 is one of three DVL homologous proteins (DVL1-3) widely expressed in embryonic development and in the adult central nervous system. Dishevelled proteins are a necessary component of the Wnt and planar cell polarity developmental signaling pathways. Overexpression of DVL1 has been linked to prostate and breast cancers through the Wnt signaling pathway. There are two isoforms of DVL1: 75 kDa and 73 kDa.