DVL1 Monoclonal antibody

DVL1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1A2A5

Cat no : 67672-1-Ig

Synonyms

Dishevelled 1, DSH homolog 1, DVL, DVL1

---

Validation Data Gallery

• 

  WB analysis using 67672-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67672-1-Ig (DVL1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of ROS1728 using 67672-1-Ig

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 67672-1-Ig (DVL1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NCCIT using 67672-1-Ig

  NCCIT cells were subjected to SDS PAGE followed by western blot with 67672-1-Ig (DVL1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of rat colon using 67672-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 67672-1-Ig (DVL1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat colon using 67672-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 67672-1-Ig (DVL1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 67672-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 67672-1-Ig (DVL1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 67672-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 67672-1-Ig (DVL1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: HEK-293 cells, ROS1728 cells, NCCIT cells, HeLa cells, MDA-MB-231 cells
• Positive IHC detected in: rat colon tissue, mouse colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67672-1-Ig targets DVL1 in WB, IHC, ELISA applications and shows reactivity with Human, rat, mouse samples.

• Tested Reactivity: Human, rat, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: DVL1 fusion protein Ag26083
• Full Name: dishevelled, dsh homolog 1 (Drosophila)
• Calculated Molecular Weight: 75 kDa
• Observed Molecular Weight: 70-75 kDa
• GenBank Accession Number: BC050454
• Gene Symbol: DVL1
• Gene ID (NCBI): 1855
• RRID: AB_2882868
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DVL1 is one of three DVL homologous proteins (DVL1-3) widely expressed in embryonic development and in the adult central nervous system. Dishevelled proteins are a necessary component of the Wnt and planar cell polarity developmental signaling pathways. Overexpression of DVL1 has been linked to prostate and breast cancers through the Wnt signaling pathway. There are two isoforms of DVL1: 75 kDa and 73 kDa.