DUSP6 Recombinant antibody

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  WB analysis using 83356-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83356-5-RR (DUSP6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  FC experiment of HeLa using 83356-5-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug DUSP6 Recombinant antibody (83356-5-RR, Clone:240295C8) (red) or Isotype Controland (blue) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of PC-12 using 83356-5-RR

  1x10^6 PC-12 cells were intracellularly stained with 0.25 ug DUSP6 Recombinant antibody (83356-5-RR, Clone:240295C8) (red) or Isotype Controland (blue) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 83356-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83356-5-RR against Human DUSP6 were performed. The affinity constant is 3.38 nM.

DUSP6 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 83356-5-RR

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

MKP 3, Mitogen-activated protein kinase phosphatase 3, MAP kinase phosphatase 3, EC:3.1.3.16, dual specificity phosphatase 6

Formulation:  PBS and Azide

PBS and Azide

PBS Only

Conjugate:  Unconjugated

Unconjugated

Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

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Tested Applications

• Positive WB detected in: A431 cells, A549 cells, HepG2 cells, NIH/3T3 cells, PC-12 cells, mouse heart tissue, rat heart tissue
• Positive FC (Intra) detected in: HeLa cells, PC-12 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83356-5-RR targets DUSP6 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: DUSP6 fusion protein Ag23474
• Full Name: dual specificity phosphatase 6
• Calculated Molecular Weight: 42 kDa
• Observed Molecular Weight: 42-45 kDa
• GenBank Accession Number: BC003562
• Gene Symbol: DUSP6
• Gene ID (NCBI): 1848
• RRID: AB_3671013
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q16828
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Dual specificity phosphatase 6 (DUSP6) is a member of the family of mitogen-activated protein kinase (MAPK) phosphatases, which dephosphorylate and inactivate MAPK (PMID: 9858808). The MAPK pathway is an important signal transduction system that serves cellular processes, such as proliferation, differentiation, migration, and apoptosis. DUSP6 resides at chromosome location 12q22-23, which is one of the candidate loci for susceptibility to bipolar disorder and which encodes a phosphatase selective for extracellular signal-regulated kinase (ERK) (PMID: 9205128). DUSP6 inactivates extracellular signal-regulated kinase (ERK), and can act in tumor suppressive pathways, whose expression is reduced in several different types of cancer. (PMID: 21680106).