CoraLite® Plus 488-conjugated DUSP11 Polyclonal antibody

• Featured Product
• KD/KO Validated

DUSP11 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-10204

Synonyms

DUSP11, PIR1

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Validation Data Gallery

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  IF Staining of HepG2 using CL488-10204

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 DUSP11 antibody (CL488-10204) at dilution of 1:200.

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  IF Staining of A431 using CL488-10204

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite® Plus 488 DUSP11 antibody (CL488-10204) at dilution of 1:600.

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Tested Applications

• Positive IF/ICC detected in: A431 cells, HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-10204 targets DUSP11 in IF/ICC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DUSP11 fusion protein Ag0276
• Full Name: dual specificity phosphatase 11 (RNA/RNP complex 1-interacting)
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 39 kDa
• GenBank Accession Number: BC000346
• Gene Symbol: DUSP11
• Gene ID (NCBI): 8446
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

DUSP11, also known as PIR1, is a unique member of atypical DUSPs which could bind directly to RNA and possess RNA 5'-triphosphatase and diphosphatase activities. DUSP11 converts the 5' triphosphate of microRNA precursors to a 5' monophosphate, and regulates cellular noncoding RNAs levels. In addition to a catalysis towards RNA, more evidence showed that DUSP11 could also dephosphorylate proteins.