DSG2 Monoclonal antibody, PBS Only (Capture)

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  WB analysis using 68515-1-Ig (same clone as 68515-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68515-1-Ig (DSG2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68515-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 68515-1-Ig (same clone as 68515-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 68515-1-Ig (DSG2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68515-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP51241-1

  Cytometric bead array standard curve of MP51241-1, DSG2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68515-1-PBS. Detection antibody: 68515-2-PBS. Standard:Ag20633. Range: 0.391-100 ng/mL.

DSG2 Monoclonal Antibody for WB, Cytometric bead array, Indirect ELISA

Cat No. 68515-1-PBS

Clone No.1B8C3

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, Cytometric bead array, Indirect ELISA

Synonyms

Desmoglein-2, Desmoglein 2, CDHF5, Cadherin family member 5, ARVD10

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Product Information

68515-1-PBS targets DSG2 as part of a matched antibody pair:

MP51241-1: 68515-1-PBS capture and 68515-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: DSG2 fusion protein Ag20633
• Full Name: desmoglein 2
• Calculated Molecular Weight: 1118 aa, 122 kDa
• Observed Molecular Weight: 145-150 kDa
• GenBank Accession Number: BC099655
• Gene Symbol: DSG2
• Gene ID (NCBI): 1829
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q14126
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. Desmosomal cadherins, consisting of four desmogleins (DSG1-4) and three desmocollins (DSC1-3) in humans, mediate adhesion through calcium-dependent homophilic/heterophilic interactions. DSG2 is a single-pass transmembrane glycoprotein that is widely expressed in epithelial and non-epithelial tissues, such as the intestine, epidermis, testis, and heart (PMID:21715983). Defects in DSG2 are the cause of familial arrhythmogenic right ventricular dysplasia type 10 (ARVD10), and genetic variations in DSG2 are the cause of susceptibility to cardiomyopathy dilated type 1BB (CMD1BB).