DSG2 Monoclonal antibody, PBS Only

DSG2 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B8C3

Cat no : 68515-1-PBS

Synonyms

ARVC10, ARVD10, Cadherin family member 5, CDHF5, desmoglein 2, DSG2, HDGC

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Validation Data Gallery

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  WB analysis using 68515-1-Ig (same clone as 68515-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68515-1-Ig (DSG2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68515-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 68515-1-Ig (same clone as 68515-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 68515-1-Ig (DSG2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68515-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68515-1-PBS targets DSG2 in WB, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: DSG2 fusion protein Ag20633
• Full Name: desmoglein 2
• Calculated Molecular Weight: 1118 aa, 122 kDa
• Observed Molecular Weight: 145-150 kDa
• GenBank Accession Number: BC099655
• Gene Symbol: DSG2
• Gene ID (NCBI): 1829
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. Desmosomal cadherins, consisting of four desmogleins (DSG1-4) and three desmocollins (DSC1-3) in humans, mediate adhesion through calcium-dependent homophilic/heterophilic interactions. DSG2 is a single-pass transmembrane glycoprotein that is widely expressed in epithelial and non-epithelial tissues, such as the intestine, epidermis, testis, and heart (PMID:21715983). Defects in DSG2 are the cause of familial arrhythmogenic right ventricular dysplasia type 10 (ARVD10), and genetic variations in DSG2 are the cause of susceptibility to cardiomyopathy dilated type 1BB (CMD1BB).