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DSG2 Monoclonal antibody

DSG2 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1B8C3

Cat no : 68515-1-Ig

Synonyms

ARVC10, ARVD10, Cadherin family member 5, CDHF5, desmoglein 2, DSG2, HDGC

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DSG2 Monoclonal antibody (68515-1-Ig)

    WB analysis using 68515-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68515-1-Ig (DSG2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using DSG2 Monoclonal antibody (68515-1-Ig)

    WB analysis of K-562 using 68515-1-Ig

    K-562 cells were subjected to SDS PAGE followed by western blot with 68515-1-Ig (DSG2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inA431 cells, K-562 cells, A549 cells, LNCaP cells, MCF-7 cells, HeLa cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68515-1-Ig targets DSG2 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen DSG2 fusion protein Ag20633
Full Name desmoglein 2
Calculated Molecular Weight 1118 aa, 122 kDa
Observed Molecular Weight145-150 kDa
GenBank Accession NumberBC099655
Gene Symbol DSG2
Gene ID (NCBI) 1829
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. Desmosomal cadherins, consisting of four desmogleins (DSG1-4) and three desmocollins (DSC1-3) in humans, mediate adhesion through calcium-dependent homophilic/heterophilic interactions. DSG2 is a single-pass transmembrane glycoprotein that is widely expressed in epithelial and non-epithelial tissues, such as the intestine, epidermis, testis, and heart (PMID:21715983). Defects in DSG2 are the cause of familial arrhythmogenic right ventricular dysplasia type 10 (ARVD10), and genetic variations in DSG2 are the cause of susceptibility to cardiomyopathy dilated type 1BB (CMD1BB).

Protocols

Product Specific Protocols
WB protocol for DSG2 antibody 68515-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols