DR6 Monoclonal antibody, PBS Only

DR6 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3C2D10

Cat no : 66754-1-PBS

Synonyms

BM 018, CD358, Death receptor 6, DR6, TNFRSF21

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Validation Data Gallery

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  WB analysis of K-562 using 66754-1-Ig (same clone as 66754-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 66754-1-Ig (DR6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66754-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 66754-1-Ig (same clone as 66754-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66754-1-Ig (DR6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66754-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Raji using 66754-1-Ig (same clone as 66754-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 66754-1-Ig (DR6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66754-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Daudi using 66754-1-Ig (same clone as 66754-1-PBS)

  Daudi cells were subjected to SDS PAGE followed by western blot with 66754-1-Ig (DR6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66754-1-PBS in a different storage buffer formulation.

• 

  WB analysis of THP-1 using 66754-1-Ig (same clone as 66754-1-PBS)

  THP-1 cells were subjected to SDS PAGE followed by western blot with 66754-1-Ig (DR6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66754-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 66754-1-Ig (same clone as 66754-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DR6 antibody (66754-1-Ig, Clone: 3C2D10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66754-1-PBS in a different storage buffer formulation.

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Product Information

66754-1-PBS targets DR6 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: DR6 fusion protein Ag14327
• Full Name: tumor necrosis factor receptor superfamily, member 21
• Calculated Molecular Weight: 655 aa, 72 kDa
• Observed Molecular Weight: 69 kDa
• GenBank Accession Number: BC021572
• Gene Symbol: DR6
• Gene ID (NCBI): 27242
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

DR6 (also known as TNFRSF21 or CD358) is an orphan receptor of the tumor necrosis factor (TNF) receptor superfamily. It controls cell death and differentiation in a cell-autonomous manner in different cell types including neurons, oligodendrocytes, endothelial cells, and immune cells (PMID: 29459438). DR6 has been shown to activate NF-κB and JNK, and induce cell apoptosis (PMID: 9714541). DR6 interacts with TRADD, which is known to mediate signal transduction of TNF receptors. It has been reported that APP binds DR6 to trigger axon pruning and neuron death via distinct caspases, indicating that DR6 might be involved in Alzheimer's disease (PMID: 19225519).