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DR6 Monoclonal antibody

DR6 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

3C2D10

Cat no : 66754-1-Ig

Synonyms

BM 018, CD358, Death receptor 6, DR6, TNFRSF21

Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using DR6 Monoclonal antibody (66754-1-Ig)

    WB analysis of K-562 using 66754-1-Ig

    K-562 cells were subjected to SDS PAGE followed by western blot with 66754-1-Ig (DR6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using DR6 Monoclonal antibody (66754-1-Ig)

    WB analysis of Jurkat using 66754-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66754-1-Ig (DR6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Raji cells using DR6 Monoclonal antibody (66754-1-Ig)

    WB analysis of Raji using 66754-1-Ig

    Raji cells were subjected to SDS PAGE followed by western blot with 66754-1-Ig (DR6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Daudi cells using DR6 Monoclonal antibody (66754-1-Ig)

    WB analysis of Daudi using 66754-1-Ig

    Daudi cells were subjected to SDS PAGE followed by western blot with 66754-1-Ig (DR6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of THP-1 cells using DR6 Monoclonal antibody (66754-1-Ig)

    WB analysis of THP-1 using 66754-1-Ig

    THP-1 cells were subjected to SDS PAGE followed by western blot with 66754-1-Ig (DR6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using DR6 Monoclonal antibody (66754-1-Ig)

    IF Staining of HepG2 using 66754-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DR6 antibody (66754-1-Ig, Clone: 3C2D10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inK-562 cells, THP-1 cells, Jurkat cells, Raji cells, Daudi cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

66754-1-Ig targets DR6 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen DR6 fusion protein Ag14327
Full Name tumor necrosis factor receptor superfamily, member 21
Calculated Molecular Weight 655 aa, 72 kDa
Observed Molecular Weight 69 kDa
GenBank Accession NumberBC021572
Gene Symbol DR6
Gene ID (NCBI) 27242
RRIDAB_2882100
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DR6 (also known as TNFRSF21 or CD358) is an orphan receptor of the tumor necrosis factor (TNF) receptor superfamily. It controls cell death and differentiation in a cell-autonomous manner in different cell types including neurons, oligodendrocytes, endothelial cells, and immune cells (PMID: 29459438). DR6 has been shown to activate NF-κB and JNK, and induce cell apoptosis (PMID: 9714541). DR6 interacts with TRADD, which is known to mediate signal transduction of TNF receptors. It has been reported that APP binds DR6 to trigger axon pruning and neuron death via distinct caspases, indicating that DR6 might be involved in Alzheimer's disease (PMID: 19225519).

Protocols

Product Specific Protocols
WB protocol for DR6 antibody 66754-1-IgDownload protocol
IF protocol for DR6 antibody 66754-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Death Discov

Knockdown of KIF15 suppresses proliferation of prostate cancer cells and induces apoptosis through PI3K/Akt signaling pathway

Authors - Hai Bi
View Article