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DNMT3A Recombinant antibody, PBS Only

DNMT3A Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

242257A9

Cat no : 81474-6-PBS

Synonyms

EC:2.1.1.37, EC:2.1.1.-, DNA MTase HsaIIIA, DNA (cytosine-5)-methyltransferase 3A, Cysteine methyltransferase DNMT3A

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using DNMT3A Recombinant antibody (81474-6-RR)

    IF Staining of HeLa using 81474-6-RR (same clone as 81474-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DNMT3A antibody (81474-6-RR, Clone: 242257A9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 81474-6-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using DNMT3A Recombinant antibody (81474-6-RR)

    FC experiment of HepG2 using 81474-6-RR (same clone as 81474-6-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Dnmt3a Recombinant Antibody (81474-6-RR, Clone:242257A9) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 81474-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 81474-6-RR

    Affinity and Kinetic Characterization of 81474-6-RR

    Biolayer interferometry (BLl) kinetic assays of 81474-6-RR against Human DNMT3A were performed. The affinity constant is 5.22 nM.

Product Information

81474-6-PBS targets DNMT3A in IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen DNMT3A fusion protein Ag15111
Full Name DNA (cytosine-5-)-methyltransferase 3 alpha
Calculated Molecular Weight 912 aa, 102 kDa
GenBank Accession NumberBC043617
Gene Symbol DNMT3A
Gene ID (NCBI) 1788
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

DNA methylation in vertebrate animals is an epigenetic modification that is important for embryonic development, imprinting, and the inactivation of X chromosomes. DNA methylation is catalyzed by a family of DNA methyltransferases (DNMTs) that include the maintenance enzyme DNMT1 and de novo methyltransferases DNMT3a and DNMT3b. The overexpression of DNMT1, DNMT3a, and DNMT3b has been reported in various malignancies, including gastric, urothelial, and lung cancers, and may be related to tumorigenesis, tumor progression, and poor survival. Two isoforms of DNMT3a exist: the full-length DNMT3a, and the shorter form DNMT3a2 which lacks the N-terminal fragment. DNMT3a is expressed ubiquitously at low levels, while DNMT3a2 is specially expressed at high levels in embryonic stem cells and shows restricted expression in tissues known to undergo de novo methylation including testis and ovary. This antibody was raised against the N-terminal region of human DNMT3a.