ChromoTek DNMT1-Trap Agarose
The ChromoTek DNMT1-Trap Agarose consists of an anti-DNMT1 Nanobody/VHH, which is coupled to agarose beads. It can be used for the immunoprecipitation of DNMT1 proteins and their interacting factors.
Cat no : dta
Synonyms
Validation Data Gallery
Product Information
The ChromoTek DNMT1-Trap Agarose consists of an anti-DNMT1 Nanobody/VHH, which is coupled to agarose beads. It can be used for the immunoprecipitation of DNMT1 proteins and their interacting factors.
Description | Immunoprecipitation of DNMT1 proteins with anti-DNMT1 Nanobody conjugated to agarose beads. • Fast, reliable & efficient one-step immunoprecipitation • Ready-to-use • No heavy & light antibody chains • Stable under harsh washing conditions • Suitable for downstream mass spec analysis |
Applications | IP, Co-IP |
Specificity/Target | DNMT1 protein |
Binding Capacity | 2-3 ug of DNMT1 protein per 10 uL bead slurry |
Conjugate | Agarose beads; ~90 um (cross-linked 4% agarose beads) |
Elution buffer | 2x SDS-sample buffer (Lammli) |
Type | Nanobody |
Class | Recombinant |
Host | Alpaca |
Compatibility with mass spectrometry | The DNMT1-Trap is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry |
RRID | AB_2631372 |
Storage Buffer | 20% ethanol |
Storage Condition | Shipped at ambient temperature. Upon receipt store at +4°C. Stable for one year. Do not freeze! |
Documentation
SDS |
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SDS DNMT1-Trap Agarose (PDF) |
Protocol |
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Protocol DNMT1-Trap Agarose (PDF) |
Trouble shooting |
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Troubleshooting guide immunoprecipitation (IP) (PDF) |
Other Formats
DNMT1-Trap Agarose Kit |