ChromoTek DNMT1-Trap Agarose, Kit for Immunoprecipitation

Validation Data Gallery

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  The DNMT1-Trap Agarose was used to immunoprecipiate endogenous DNMT1 protein from HEK293T cells. Input (I), Flow-Through (FT), Bound (B).

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  IP of endogenous DNMT1 protein from HEK293T cells using DNMT-1 Trap Agarose followed by western blot using DNMT1 Polyclonal Antibody (24206-1-AP) and Multi-rAb HRP-Goat Anti-Rabbit Recombinant Secondary Antibody (RGAR001). Samples from the Input (I), Flow-Through (F), and Bound (B) fractions were used in the western blot analysis.

The DNMT1-Trap Agarose Kit is a ready-to-use reagent for the IP of DNMT1 proteins. It consists of an anti-DNMT1 Nanobody/VHH coupled to agarose beads, along with lysis, wash, and elution buffers to use in the IP process.

Cat No. dtak

Specificity

DNMT1

Applications

IP, Co-IP

Type

Nanobody

Conjugate

Agarose

Synonyms

DNMT1, DNA methyltransferase, AIM, CXXC9, HSN1E, DNMT, DNMT1 Trap

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Product Information

The DNMT1-Trap Agarose Kit is a ready-to-use reagent for the IP of DNMT1 proteins. It consists of an anti-DNMT1 Nanobody/VHH coupled to agarose beads, along with lysis, wash, and elution buffers to use in the IP process.

• Description: Immunoprecipitation of DNMT1 proteins with anti-DNMT1 Nanobody conjugated to agarose beads.;

  • Fast, reliable & efficient one-step immunoprecipitation;

  • Ready-to-use;

  • No heavy & light antibody chains;

  • Stable under harsh washing conditions;

  • Suitable for downstream mass spec analysis;

• Applications: IP, Co-IP
• Specificity/Target: DNMT1
• Binding Capacity: 2-3 ug of DNMT1 per 10 uL bead slurry
• Conjugate: Agarose beads; ~90 um (cross-linked 4% agarose beads)
• Elution buffer: 2x SDS-sample buffer (Lammli)
• Type: Nanobody
• Class: Recombinant
• Host: Alpaca
• Compatibility with mass spectrometry: The DNMT1-Trap is optimized for on-bead digestion. For the application note, please click here:; On-bead digest protocol for mass spectrometry
• RRID: AB_2631372
• Storage Buffer: 20% ethanol
• Storage Condition: Shipped at ambient temperature. Upon receipt store at +4°C. Stable for one year. DO not freeze!

Kit components

• Component: Description
• DNMT1-Trap Agarose: 20 reactions (500 µl)
• Lysis buffer: Optimized for cytoplasmic proteins and mammalian cell lysis
• RIPA buffer: Optimized for nuclear/chromatin proteins and mammalian cell lysis
• Wash buffer: Removal of unwanted proteins, peptides, etc.
• Dilution buffer: Dilution of cell lysate
• Elution buffer: For acidic elution

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