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CoraLite®594-conjugated DNASE1L3 Monoclonal antibody

DNASE1L3 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1A4D4

Cat no : CL594-67041

Synonyms

DNase gamma, DNAS1L3, DHP2, Deoxyribonuclease I-like 3, Deoxyribonuclease gamma

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite®594-conjugated DNASE1L3 Monoclonal antibo (CL594-67041)

    IF Staining of HepG2 using CL594-67041

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594 DNASE1L3 antibody (CL594-67041, Clone: 1A4D4 ) at dilution of 1:200, CL488-Phalloidin (green).

  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite®594-conjugated DNASE1L3 Monoclonal antibo (CL594-67041)

    FC experiment of HepG2 using CL594-67041

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human DNASE1L3 (CL594-67041, Clone:1A4D4) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL594-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67041 targets DNASE1L3 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen DNASE1L3 fusion protein Ag28187
Full Name deoxyribonuclease I-like 3
Calculated Molecular Weight 305 aa, 36 kDa
Observed Molecular Weight 33-38 kDa
GenBank Accession NumberBC015831
Gene Symbol DNASE1L3
Gene ID (NCBI) 1776
RRIDAB_2920078
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Deoxyribonuclease 1-like 3 (DNASE1L3, DNase gamma, DNase Y, LS-DNase) is member of a DNASE1 protein family that is identified by similar biochemical properties such as Ca2+/Mg2+- dependency and an optimal pH of about 7.0 as well as by a high similarity in their nucleic acid and amino acid sequences [PMID:15796714]. It is an endonuclease and cleaves double-stranded DNA (and single-stranded DNA) producing 3′-OH/5′-P ends, which is Ca2+/Mg2+-dependent and inhibited by Zn2+, but not by monomeric actin [PMID:9665719]. In addition, it has been suggested to be involved in apoptotic DNA fragmentation of thymocytes' neuronal PC12 cells, C2C12 myoblasts and of cell lines expressing the recombinant nuclease [PMID: 7957253, 9307016]

Protocols

Product Specific Protocols
IF protocol for CL594 DNASE1L3 antibody CL594-67041Download protocol
FC protocol for CL594 DNASE1L3 antibody CL594-67041Download protocol
Standard Protocols
Click here to view our Standard Protocols