DNAJB4 Monoclonal antibody, PBS Only

DNAJB4 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig, rabbit, mouse

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1F3H7

Cat no : 67966-1-PBS

Synonyms

DjB4, DNAJB4, DNAJW, Heat shock protein 40 homolog, HLJ1, HSP40 homolog, Human liver DnaJ like protein

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Validation Data Gallery

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  WB analysis using 67966-1-Ig (same clone as 67966-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67966-1-Ig (DNAJB4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67966-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse heart using 67966-1-Ig (same clone as 67966-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 67966-1-Ig (DNAJB4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67966-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 67966-1-Ig (same clone as 67966-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67966-1-Ig (DNAJB4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67966-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 67966-1-Ig (same clone as 67966-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67966-1-Ig (DNAJB4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67966-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 67966-1-Ig (same clone as 67966-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using DNAJB4 antibody (67966-1-Ig, Clone: 1F3H7 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67966-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67966-1-PBS targets DNAJB4 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, pig, rabbit, mouse samples.

• Tested Reactivity: Human, pig, rabbit, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: DNAJB4 fusion protein Ag3756
• Full Name: DnaJ (Hsp40) homolog, subfamily B, member 4
• Calculated Molecular Weight: 38 kDa
• Observed Molecular Weight: 38 kDa
• GenBank Accession Number: BC034721
• Gene Symbol: DNAJB4
• Gene ID (NCBI): 11080
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.