Various lysates were subjected to SDS PAGE followed by western blot with 67966-1-Ig (DNAJB4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 67966-1-Ig (DNAJB4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of mouse heart using 67966-1-Ig
mouse heart tissue were subjected to SDS PAGE followed by western blot with 67966-1-Ig (DNAJB4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
mouse heart tissue were subjected to SDS PAGE followed by western blot with 67966-1-Ig (DNAJB4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
IHC staining of human pancreas cancer using 67966-1-Ig
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67966-1-Ig (DNAJB4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67966-1-Ig (DNAJB4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas cancer using 67966-1-Ig
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67966-1-Ig (DNAJB4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67966-1-Ig (DNAJB4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of A431 using 67966-1-Ig
Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using DNAJB4 antibody (67966-1-Ig, Clone: 1F3H7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).
Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using DNAJB4 antibody (67966-1-Ig, Clone: 1F3H7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).
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human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
A431 cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:2000-1:10000
Immunohistochemistry (IHC)
IHC : 1:250-1:1000
Immunofluorescence (IF)/ICC
IF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
67966-1-Ig targets DNAJB4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, pig, rabbit, mouse samples.
Various lysates were subjected to SDS PAGE followed by western blot with 67966-1-Ig (DNAJB4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of mouse heart using 67966-1-Ig
mouse heart tissue were subjected to SDS PAGE followed by western blot with 67966-1-Ig (DNAJB4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human pancreas cancer using 67966-1-Ig
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67966-1-Ig (DNAJB4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas cancer using 67966-1-Ig
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67966-1-Ig (DNAJB4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF/ICC Figures
IF Staining of A431 using 67966-1-Ig
Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using DNAJB4 antibody (67966-1-Ig, Clone: 1F3H7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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