DNAJB1 Monoclonal antibody, PBS Only

DNAJB1 Monoclonal Antibody for Indirect ELISA, WB

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

Indirect ELISA, WB

Conjugate

Unconjugated

CloneNo.

1E8E10

Cat no : 67422-1-PBS

Synonyms

hDj-1, Hdj1, hDj 1, DNAJ1, DnaJ protein homolog 1

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Validation Data Gallery

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  WB analysis using 67422-1-Ig (same clone as 67422-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67422-1-Ig (DNAJB1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67422-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 67422-1-Ig (same clone as 67422-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human DNAJB1 (67422-1-Ig, Clone:1E8E10) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 67422-1-PBS in a different storage buffer formulation.

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Product Information

67422-1-PBS targets DNAJB1 in Indirect ELISA, WB applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: DNAJB1 fusion protein Ag3830
• Full Name: DnaJ (Hsp40) homolog, subfamily B, member 1
• Calculated Molecular Weight: 340 aa, 40 kDa
• Observed Molecular Weight: 38 kDa
• GenBank Accession Number: BC019827
• Gene Symbol: DNAJB1
• Gene ID (NCBI): 3337
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

DNAJB1 is a member of the heat shock 40 protein family and has been involved in a variety of cellular processes, including the proteasome pathway, endoplasmic reticulum stress and viral infection. DNAJB1-PRKACA gene fusions have been considered diagnostic for fibrolamellar hepatocellular carcinoma.