DNA-PKcs Polyclonal antibody, PBS Only

DNA-PKcs Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 28534-1-PBS

Synonyms

---

Validation Data Gallery

• 

  WB analysis using 28534-1-AP (same clone as 28534-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 28534-1-AP (DNA-PKcs antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28534-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 28534-1-AP (same clone as 28534-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 28534-1-AP (DNA-PKcs antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28534-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 28534-1-AP (same clone as 28534-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 28534-1-AP (DNA-PKcs antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28534-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 28534-1-AP (same clone as 28534-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 28534-1-AP (DNA-PKcs antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28534-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 28534-1-AP (same clone as 28534-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 28534-1-AP (DNA-PKcs antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28534-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 28534-1-AP (same clone as 28534-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 28534-1-AP (DNA-PKcs antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 28534-1-PBS in a different storage buffer formulation.

---

Product Information

28534-1-PBS targets DNA-PKcs in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DNA-PKcs fusion protein Ag29100
• Full Name: protein kinase, DNA-activated, catalytic polypeptide
• Calculated Molecular Weight: 469 kDa
• Observed Molecular Weight: 350-460 kDa
• GenBank Accession Number: NM_006904
• Gene Symbol: PRKDC
• Gene ID (NCBI): 5591
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

PRKDC, also named as HYRC, HYRC1, DNPK1 and p460, belongs to the PI3/PI4-kinase family. PRKDC is a serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA nonhomologous end joining (NHEJ), PRKDC is required for double-strand break (DSB) repair and V(D)J recombination. PRKDC must be bound to DNA to express its catalytic properties. It promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis (DCLRE1C). It is required to protect and align broken ends of DNA. PRKDC may also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. It is found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion. It also involved in modulation of transcription. It recognizes the substrate consensus sequence [ST]-Q. PRKDC phosphorylates 'Ser-139' of histone variant H2AX/H2AFX, thereby regulating DNA damage response mechanism. It phosphorylates DCLRE1C, c-Abl/ABL1, histone H1, HSPCA, c-jun/JUN, p53/TP53, PARP1, POU2F1, DHX9, SRF, XRCC1, XRCC1, XRCC4, XRCC5, XRCC6, WRN, c-myc/MYC and RFA2. The antibody recognizes the C-term of PRKDC.