DNA-PKcs Polyclonal antibody

DNA-PKcs Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 28534-1-AP

Synonyms

DNA PK catalytic subunit, DNA PKcs, DNAPK, DNPK1, HYRC, HYRC1, p350, p460, PRKDC, XRCC7

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Validation Data Gallery

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  WB analysis using 28534-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28534-1-AP (DNA-PKcs antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 28534-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 28534-1-AP (DNA-PKcs antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 28534-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 28534-1-AP (DNA-PKcs antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 28534-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 28534-1-AP (DNA-PKcs antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 28534-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 28534-1-AP (DNA-PKcs antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 28534-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 28534-1-AP (DNA-PKcs antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, MCF-7 cells
• Positive IHC detected in: mouse testis tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

28534-1-AP targets DNA-PKcs in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DNA-PKcs fusion protein Ag29100
• Full Name: protein kinase, DNA-activated, catalytic polypeptide
• Calculated Molecular Weight: 469 kDa
• Observed Molecular Weight: 350-460 kDa
• GenBank Accession Number: NM_006904
• Gene Symbol: PRKDC
• Gene ID (NCBI): 5591
• RRID: AB_2881165
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PRKDC, also named as HYRC, HYRC1, DNPK1 and p460, belongs to the PI3/PI4-kinase family. PRKDC is a serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA nonhomologous end joining (NHEJ), PRKDC is required for double-strand break (DSB) repair and V(D)J recombination. PRKDC must be bound to DNA to express its catalytic properties. It promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis (DCLRE1C). It is required to protect and align broken ends of DNA. PRKDC may also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. It is found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion. It also involved in modulation of transcription. It recognizes the substrate consensus sequence [ST]-Q. PRKDC phosphorylates 'Ser-139' of histone variant H2AX/H2AFX, thereby regulating DNA damage response mechanism. It phosphorylates DCLRE1C, c-Abl/ABL1, histone H1, HSPCA, c-jun/JUN, p53/TP53, PARP1, POU2F1, DHX9, SRF, XRCC1, XRCC1, XRCC4, XRCC5, XRCC6, WRN, c-myc/MYC and RFA2. The antibody recognizes the C-term of PRKDC.

Publications

Species	Application	Title
human	WB	Sci Adv; SMYD2 inhibition-mediated hypomethylation of Ku70 contributes to impaired nonhomologous end joining repair and antitumor immunity; Authors - Ming Tang