DMXL2 Monoclonal antibody, PBS Only

DMXL2 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1F9A8

Cat no : 66891-2-PBS

Synonyms

Rabconnectin 3, KIAA0856, DmX-like protein 2, DmX like protein 2, Dmx like 2

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Validation Data Gallery

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  WB analysis of mouse cerebellum using 66891-2-Ig (same clone as 66891-2-PBS)

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 66891-2-Ig (DMXL2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66891-2-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 66891-2-Ig (same clone as 66891-2-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66891-2-Ig (DMXL2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66891-2-PBS in a different storage buffer formulation.

• 

  WB analysis of rat brain using 66891-2-Ig (same clone as 66891-2-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66891-2-Ig (DMXL2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66891-2-PBS in a different storage buffer formulation.

• 

  IF Staining of Jurkat using 66891-2-Ig (same clone as 66891-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using DMXL2 antibody (66891-2-Ig, Clone: 1F9A8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66891-2-PBS in a different storage buffer formulation.

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Product Information

66891-2-PBS targets DMXL2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: DMXL2 fusion protein Ag19861
• Full Name: Dmx-like 2
• Calculated Molecular Weight: 3036 aa, 340 kDa
• Observed Molecular Weight: 340 kDa
• GenBank Accession Number: BC140781
• Gene Symbol: DMXL2
• Gene ID (NCBI): 23312
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

DMXL2, also named as rabconnectin-3, is a functional regulator of mammalian notch signaling with WD domains. Rabconnectin-3 is abundantly expressed in the brain where it is enriched in the synaptic vesicle fraction. Immunofluorescence and immunoelectron microscopy revealed that rabconnectin-3 was concentrated on synaptic vesicles at synapses. It may serve as a scaffold molecule for both Rab3 GEP and GAP on synaptic vesicles.