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DMXL2 Monoclonal antibody

DMXL2 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1F9A8

Cat no : 66891-2-Ig

Synonyms

Rabconnectin 3, KIAA0856, DmX-like protein 2, DmX like protein 2, Dmx like 2

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse cerebellum tissue using DMXL2 Monoclonal antibody (66891-2-Ig)

    WB analysis of mouse cerebellum using 66891-2-Ig

    mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 66891-2-Ig (DMXL2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using DMXL2 Monoclonal antibody (66891-2-Ig)

    WB analysis of Jurkat using 66891-2-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66891-2-Ig (DMXL2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using DMXL2 Monoclonal antibody (66891-2-Ig)

    WB analysis of rat brain using 66891-2-Ig

    rat brain tissue were subjected to SDS PAGE followed by western blot with 66891-2-Ig (DMXL2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using DMXL2 Monoclonal antibody (66891-2-Ig)

    IF Staining of Jurkat using 66891-2-Ig

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using DMXL2 antibody (66891-2-Ig, Clone: 1F9A8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

Tested Applications

Positive WB detected inmouse cerebellum tissue, Jurkat cells, rat brain tissue
Positive IF/ICC detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66891-2-Ig targets DMXL2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen DMXL2 fusion protein Ag19861
Full Name Dmx-like 2
Calculated Molecular Weight 3036 aa, 340 kDa
Observed Molecular Weight 340 kDa
GenBank Accession NumberBC140781
Gene Symbol DMXL2
Gene ID (NCBI) 23312
RRIDAB_2882221
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DMXL2, also named as rabconnectin-3, is a functional regulator of mammalian notch signaling with WD domains. Rabconnectin-3 is abundantly expressed in the brain where it is enriched in the synaptic vesicle fraction. Immunofluorescence and immunoelectron microscopy revealed that rabconnectin-3 was concentrated on synaptic vesicles at synapses. It may serve as a scaffold molecule for both Rab3 GEP and GAP on synaptic vesicles.

Protocols

Product Specific Protocols
WB protocol for DMXL2 antibody 66891-2-IgDownload protocol
IF protocol for DMXL2 antibody 66891-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols