DMT1 Recombinant antibody

DMT1 Uni-rAb^TM Recombinant Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

CloneNo.

1I13

Reviews (1)

Cat no : 81609-1-RR

Synonyms

SLC11A2, OK/SW-cl.20, Natural resistance-associated macrophage protein 2, DMT-1, DCT1

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Validation Data Gallery

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  WB analysis using 81609-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 81609-1-RR (DMT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 81609-1-RR

  IP result of anti-DMT1 (IP:81609-1-RR, 4ug; Detection:81609-1-RR 1:2000) with HEK-293 cells lysate 1480 ug.

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  Affinity and Kinetic Characterization of 81609-1-RR

  Biolayer interferometry (BLl) kinetic assays of 81609-1-RR against Human DMT1 were performed. The affinity constant is 1.93 nM.

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, HEK-293 cells, HuH-7 cells, Caco-2 cells, Neuro-2a cells, HSC-T6 cells
• Positive IP detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

81609-1-RR targets DMT1 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: DMT1 fusion protein Ag14314
• Full Name: solute carrier family 11 (proton-coupled divalent metal ion transporters), member 2
• Calculated Molecular Weight: 568 aa, 62 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC002592
• Gene Symbol: DMT1
• Gene ID (NCBI): 4891
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SLC11A2 (also known as DMT1, Nramp2, and DCT1) is a member of the divalent cation transporters that plays a central role in iron homeostasis. SLC11A2 is widely expressed in many tissues including brain, kidney, testis, duodenum and placenta. As a membrane protein, SLC11A2 is localized on the apical membrane of enterocytes as well as in transferrin-cycle endosomes. Four isoforms of SLC11A2 exist due to the alternative splicing. They differ at the NH2 and COOH termini but share a common central domain. A variety of molecular weights of SLC11A2 in western blot analysis, ranging from 50 to 100 kDa, has been reported in different cells and species. The differences in molecular weights may be attributed to the level of glycosylation, proteolysis, or the membrane protein itself. This antibody detected various forms of SLC11A2 of 45-100 kDa.

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Amandine (Verified Customer) (02-06-2024)

With 20ug of protein denatured or not, 1H30 RT 1/10000, secondary antibody 2h 1/5000. Chemiluminescence revelation, 6min of exposition

• Applications: Western Blot
• Primary Antibody Dilution: 1/10000
• Cell Tissue Type: ImCC

DMT1 Antibody Western Blot validation (1/10000 dilution) in ImCC (Cat no:81609-1-RR)