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DMT1 Recombinant antibody, PBS Only

DMT1 Uni-rAbTM Recombinant Antibody for Indirect ELISA, IP, WB

Host / Isotype

Rabbit / IgG

Reactivity

rat, mouse, human

Applications

Indirect ELISA, IP, WB

Conjugate

Unconjugated

CloneNo.

1I13

Cat no : 81609-1-PBS

Synonyms

SLC11A2, OK/SW-cl.20, Natural resistance-associated macrophage protein 2, DMT-1, DCT1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DMT1 Recombinant antibody (81609-1-RR)

    WB analysis using 81609-1-RR (same clone as 81609-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81609-1-RR (DMT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81609-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using DMT1 Recombinant antibody (81609-1-RR)

    IP experiment of HEK-293 using 81609-1-RR (same clone as 81609-1-PBS)

    IP result of anti-DMT1 (IP:81609-1-RR, 4ug; Detection:81609-1-RR 1:2000) with HEK-293 cells lysate 1480 ug. This data was developed using the same antibody clone with 81609-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 81609-1-RR

    Affinity and Kinetic Characterization of 81609-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81609-1-RR against Human DMT1 were performed. The affinity constant is 1.93 nM.

Product Information

81609-1-PBS targets DMT1 in Indirect ELISA, IP, WB applications and shows reactivity with rat, mouse, human samples.

Tested Reactivity rat, mouse, human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen DMT1 fusion protein Ag14314
Full Name solute carrier family 11 (proton-coupled divalent metal ion transporters), member 2
Calculated Molecular Weight 568 aa, 62 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC002592
Gene Symbol DMT1
Gene ID (NCBI) 4891
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

SLC11A2 (also known as DMT1, Nramp2, and DCT1) is a member of the divalent cation transporters that plays a central role in iron homeostasis. SLC11A2 is widely expressed in many tissues including brain, kidney, testis, duodenum and placenta. As a membrane protein, SLC11A2 is localized on the apical membrane of enterocytes as well as in transferrin-cycle endosomes. Four isoforms of SLC11A2 exist due to the alternative splicing. They differ at the NH2 and COOH termini but share a common central domain. A variety of molecular weights of SLC11A2 in western blot analysis, ranging from 50 to 100 kDa, has been reported in different cells and species. The differences in molecular weights may be attributed to the level of glycosylation, proteolysis, or the membrane protein itself. This antibody detected various forms of SLC11A2 of 45-100 kDa.