CoraLite® Plus 647-conjugated DLL3 Polyclonal antibody

DLL3 Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat no : CL647-25535

Synonyms

delta like 3 (Drosophila), Delta like protein 3, Delta3, DLL3, Drosophila Delta homolog 3, SCDO1



Tested Applications

Positive IF detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-25535 targets DLL3 in IF applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DLL3 fusion protein Ag21965
Full Name delta-like 3 (Drosophila)
Calculated Molecular Weight 65 kDa
Observed Molecular Weight 65 kDa
GenBank Accession NumberBC000218
Gene Symbol DLL3
Gene ID (NCBI) 10683
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The Delta-Notch pathway is an evolutionarily conserved signaling pathway which controls a broad range of developmental processes including cell fate determination, terminal differentiation and proliferation (PMID: 22353464). In mammals, four Notch receptors (NOTCH1-4) and five activating canonical ligands (JAGGED1, JAGGED2, DLL1, DLL3 and DLL4) have been described (PMID: 22353464). DLL3 is an inhibitory ligand of the Notch signaling pathway that is predominantly localizes to the Golgi apparatus (PMID: 17664336) in normal condition. Normal tissue expression of DLL3 is highest in fetal brain, and DLL3 plays a key role in somitogenesis in the paraxial mesoderm (PMID: 26311731). It has been reported that DLL3 is expressed on the surface of tumor cells of small cell lung cancer (SCLC) and high-grade neuroendocrine carcinomas (LCNEC) and has emerged as a novel therapeutic target (PMID: 26311731; 28487384).

Protocols

Product Specific Protocols
IF protocol for CL Plus 647 DLL3 antibody CL647-25535Download protocol
Standard Protocols
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