CoraLite® Plus 488-conjugated DLC1 Monoclonal antibody

DLC1 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1G10H12

Cat no : CL488-66894

Synonyms

ARHGAP7, deleted in liver cancer 1, DLC 1, DLC1, FLJ21120, HP, HP protein, KIAA1723, p122 RhoGAP, STARD12

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Validation Data Gallery

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  IF Staining of A375 using CL488-66894

  Immunofluorescent analysis of (-20°C Methanol) fixed A375 cells using CoraLite® Plus 488 DLC1 antibody (CL488-66894, Clone: 1G10H12 ) at dilution of 1:200.

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Tested Applications

• Positive IF/ICC detected in: A375 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66894 targets DLC1 in IF/ICC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: DLC1 fusion protein Ag24404
• Full Name: deleted in liver cancer 1
• Calculated Molecular Weight: 171 kDa
• Observed Molecular Weight: 115 kDa
• GenBank Accession Number: BC054511
• Gene Symbol: DLC1
• Gene ID (NCBI): 10395
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Deleted in Liver Cancer 1 (DLC1) is a RhoGAP-containing tumor suppressor that inhibits angiogenesis by repressing VEGF production in epithelial cells. DLC1 is often down-regulated in a variety of cancers, such as those of the liver, lung, breast, stomach, brain, colon and prostate due to either genomic deletion or aberrant DNA methylation.