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CoraLite® Plus 488-conjugated DLAT Monoclonal antibody

DLAT Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1F2B9

Cat no : CL488-68303

Synonyms

PDC-E2, ODP2, EC:2.3.1.12, DLTA, Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated DLAT Monoclonal anti (CL488-68303)

    IF Staining of HepG2 using CL488-68303

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 DLAT antibody (CL488-68303, Clone: 1F2B9 ) at dilution of 1:400.

  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite® Plus 488-conjugated DLAT Monoclonal anti (CL488-68303)

    FC experiment of HepG2 using CL488-68303

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated DLAT Monoclonal antibody (CL488-68303, Clone:1F2B9)(red), or 0.4 ug CoraLite® Plus 488 Mouse IgG2b isotype control (11B8C4) (CL488-66360-3, Clone: 11B8C4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68303 targets DLAT in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen DLAT fusion protein Ag4226
Full Name dihydrolipoamide S-acetyltransferase
Calculated Molecular Weight 647 aa, 70 kDa
Observed Molecular Weight 69-70 kDa
GenBank Accession NumberBC039084
Gene Symbol DLAT
Gene ID (NCBI) 1737
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 DLAT antibody CL488-68303Download protocol
Standard Protocols
Click here to view our Standard Protocols