DLAT Monoclonal antibody, PBS Only

DLAT Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1F2B9

Cat no : 68303-1-PBS

Synonyms

DLAT, DLTA, PBC, PDC E2, PDCE2

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Validation Data Gallery

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  WB analysis using 68303-1-Ig (same clone as 68303-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68303-1-Ig (DLAT antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68303-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 68303-1-Ig (same clone as 68303-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68303-1-Ig (DLAT antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68303-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 68303-1-Ig (same clone as 68303-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68303-1-Ig (DLAT antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68303-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 68303-1-Ig (same clone as 68303-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68303-1-Ig (DLAT antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68303-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 68303-1-Ig (same clone as 68303-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68303-1-Ig (DLAT antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68303-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 68303-1-Ig (same clone as 68303-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DLAT antibody (68303-1-Ig, Clone: 1F2B9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68303-1-PBS in a different storage buffer formulation.

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Product Information

68303-1-PBS targets DLAT in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: DLAT fusion protein Ag4226
• Full Name: dihydrolipoamide S-acetyltransferase
• Calculated Molecular Weight: 647 aa, 70 kDa
• Observed Molecular Weight: 69-70 kDa
• GenBank Accession Number: BC039084
• Gene Symbol: DLAT
• Gene ID (NCBI): 1737
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.